H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis
H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis
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DOI：
10.3389/fmolb.2021.635470
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发表时间：
2021-03-11
影响因子：
5
通讯作者：
Pei, Yanxi
中科院分区：
文献类型：
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作者：
Du, Xinzhe;Jin, Zhuping;Pei, Yanxi
硫化氢（H2S）与一氧化氮、氧化碳一起是一种气体递质，参与植物的生长发育以及生物和非生物的抗逆性。在之前的研究中，我们报道了丝裂原活化蛋白激酶，特别是MPK4，是H2S参与缓解冷胁迫的重要下游组分；然而，潜在的机制尚不清楚。在这项研究中，我们确定H2S缓解冷胁迫的能力在mpk4突变体中受损，但在上游的mek2和crlk1突变体中没有受损。MPK4基本过硫化，NaHS （H2S供体）进一步提高了MPK4的过硫化水平。MEK2未被H2S过硫化。NaHS处理使MPK4活性水平提高了近10倍。MPK4的8个半胱氨酸残基分别发生位点突变后，其过硫化信号并未消失。综上所述，我们的研究结果表明H2S通过过硫化MPK4和提高MPK4激酶活性直接缓解了冷胁迫。
Hydrogen sulfide (H2S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of H2S involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of H2S to alleviate cold stress is impaired in mpk4 mutants, but not in the upstream mek2 and crlk1 mutants. MPK4 was basically persulfidated, and NaHS (H2S donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by H2S. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that H2S alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity.