Manifold anomalies in gene expression in a vineyard isolate of Saccharomyces cerevisiae revealed by DNA microarray analysis
Manifold anomalies in gene expression in a vineyard isolate of Saccharomyces cerevisiae revealed by DNA microarray analysis
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DOI：
10.1073/pnas.210395297
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Hartl, DL
中科院分区：
文献类型：
--
作者：
Cavalieri, D;Townsend, JP;Hartl, DL
全基因组转录谱分析在进化生物学中具有重要的应用，用于分析自然群体中基因表达的数量变异的等位基因的杂合性程度。我们已经使用DNA微阵列分析，以研究全球模式的转录在同宗配合的酿酒酵母菌株分离的酿酒葡萄在托斯卡纳葡萄园，沿着的二倍体后代孢子形成后获得。亲本菌株的三个非连锁位点显示2：2分离（杂合性）。一个决定了对三氟亮氨酸的抗性;另一个决定了对硫酸铜的抗性;第三个与观察到的具有类似于细丝的脊状表面的菌落的形态学表型相关。对具有细丝状和光滑菌落表型的后代的全局表达分析显示，378个基因（基因组的6%）的转录差异大于2倍。大量过表达的基因在氨基酸生物合成（特别是蛋氨酸）和硫或氮同化途径中起作用，而许多低表达的基因是氨基酸渗透酶。这些氨基酸代谢的大规模变化分离为由单个基因或少量基因引起的一系列性状。我们的结论是，自然的葡萄园人口的酿酒酵母可以窝藏等位基因，导致基因表达的全球模式的巨大变化。因此，在自然群体中的表达变异的研究，没有伴随的分离分析，可能会给出一个错误的图片的分离基因的数量的变化。
Genome-wide transcriptional profiling has important applications in evolutionary biology for assaying the extent of heterozygosity for alleles showing quantitative variation in gene expression in natural populations. We have used DNA microarray analysis to study the global pattern of transcription in a homothallic strain of Saccharomyces cerevisiae isolated from wine grapes in a Tuscan vineyard, along with the diploid progeny obtained after sporulation. The parental strain shows 2:2 segregation (heterozygosity) for three unlinked loci. One determines resistance to trifluoroleucine; another, resistance to copper sulfate; and the third is associated with a morphological phenotype observed as colonies with a ridged surface resembling a filigree, Global expression analysis of the progeny with the filigreed and smooth colony phenotypes revealed a greater than 2-fold difference in transcription for 378 genes (6% of the genome). A large number of the overexpressed genes function in pathways of amino acid biosynthesis (particularly methionine) and sulfur or nitrogen assimilation, whereas many of the underexpressed genes are amino acid permeases, These wholesale changes in amino acid metabolism segregate as a suite of traits resulting from a single gene or a small number of genes. We conclude that natural vineyard populations of S, cerevisiae can harbor alleles that cause massive alterations in the global patterns of gene expression. Hence, studies of expression variation in natural populations, without accompanying segregation analysis, may give a false picture of the number of segregating genes underlying the variation.