Metastatic mouse melanoma cells release collagen-gelatin degrading metalloproteinases as components of shed membrane vesicles.
Metastatic mouse melanoma cells release collagen-gelatin degrading metalloproteinases as components of shed membrane vesicles.
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转移性小鼠黑色素瘤细胞释放胶原明胶降解金属蛋白酶作为脱落膜囊泡的成分。
DOI：
10.1016/0304-4165(87)90091-2
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
B. Lane
中科院分区：
文献类型：
--
作者：
S. Zucker;J. Wieman;R. Lysik;D. Wilkie;N. Ramamurthy;B. Lane
这项研究的目的是比较从癌细胞细胞器和胞浆中分离出来的胶原-明胶降解酶和癌细胞释放的金属蛋白酶。为此，用蔗糖密度梯度离心法分离转移的小鼠黑色素瘤细胞细胞器，并用天然和变性的[甲基-3 H]胶原底物检测金属蛋白酶。通过硫酸铵沉淀、阴离子交换、Cancanavin A亲和层析和凝胶过滤柱层析等步骤纯化了可溶性蛋白水解酶，并用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法对其进行了鉴定。结论如下：(1)恶性黑色素瘤细胞有一种金属蛋白酶(Mr=59000)，它是作为完整的膜泡的组成部分而不是作为可溶性酶从细胞中释放到条件培养液中的；(2)肿瘤条件培养液的储存导致了低分子量自激活的可溶性金属蛋白酶的产生；(3)对这些金属蛋白酶的纯化产生了不同的胶原酶，它们具有相当的明胶溶解活性，并且胶原型金属蛋白酶的典型的合成八肽底物中的甘氨酸-Ile键具有断裂的优先位置。有人认为，在侵袭过程中，强大的蛋白酶定位于癌细胞表面，有助于结缔组织的局部破坏。
The purpose of this study has been to compare collagen-gelatin degrading enzymes isolated from cancer cell organelles and cytosol to the metalloproteinases released by cancer cells. To this end, metastatic mouse melanoma cell organelles were isolated by sucrose density gradient centrifugation and metalloproteinases were assayed using native and denatured [methyl-3H]collagen substrates. Solubilized proteinases were purified by ammonium sulfate precipitation, anion exchange, cancanavalin A affinity and gel-filtration column chromatographic procedures and characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The conclusions were as follows: (1) malignant melanoma cells have a metalloproteinase (Mr= 59 000) which is shed from cells into conditioned medium as a component of intact membrane vesicles rather than as a soluble enzyme; (2) storage of tumor-conditioned medium leads to the generation of autoactivated soluble metalloproteinases of lower molecular weight; (3) purification of these metalloproteinase species yielded variant collagenases that have considerable gelatinolytic activity and a cleavage preference site for the Gly-Ile bond in a collagen-like synthetic octapeptide substrate which is typical for collagenase-type metalloproteinases. It is proposed that localization of potent proteinases to the surface of cancer cells facilitates the local breakdown of connective tissues during the invasive process.
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大鼠子宫胶原酶的明胶分解活性。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Welgus,HG;Grant,GA;Sacchettini,JC;Roswit,WT;Jeffrey,JJ
通讯作者：
Jeffrey,JJ
影响因子：
56.9
作者：
DVORAK, HF;QUAY, SC;CARVALHO, AC
通讯作者：
CARVALHO, AC
影响因子：
3.9
作者：
Ennouri, Marwa;Petit, Laeticia;Elhouichet, Habib
通讯作者：
Elhouichet, Habib
DOI：
10.1093/jnci/75.3.517
发表时间：
1985-09
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
S. Zucker;R. Lysik;N. Ramamurthy;L. Golub;J. Wieman;D. Wilkie
通讯作者：
S. Zucker;R. Lysik;N. Ramamurthy;L. Golub;J. Wieman;D. Wilkie
大鼠子宫原胶原酶的纯化和性质。
影响因子：
3.9
作者：
Roswit,WT;Halme,J;Jeffrey,JJ
通讯作者：
Jeffrey,JJ