COMPETITION FOR OVERLAPPING SITES IN THE REGULATORY REGION OF THE DROSOPHILA GENE KRUPPEL
COMPETITION FOR OVERLAPPING SITES IN THE REGULATORY REGION OF THE DROSOPHILA GENE KRUPPEL
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DOI：
10.1126/science.1348871
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发表时间：
1992-04-03
期刊：
影响因子：
56.9
通讯作者：
JACKLE, H
中科院分区：
文献类型：
--
作者：
HOCH, M;GERWIN, N;JACKLE, H
一个730个碱基对的元件调节果蝇间隙基因Kruppel（Kr）的表达，以响应果蝇前形态原bicoid（bcd）。由knirps（kni）和tll（tll）基因编码的两种激素受体样蛋白质，特异性地与该元件结合。在体外，kni蛋白与含有同源结构域的bcd蛋白竞争结合16个碱基对的靶序列。体内实验表明，kni和tll作为bcd介导的Kr激活的竞争性阻遏物。这些结果表明一种机制，发育基因可以调节在一个激活的形态梯度拮抗阻遏物。
A 730-base pair element regulates expression of the Drosophila gap gene Kruppel (Kr) in response to the fly anterior morphogen bicoid (bcd). Two hormone receptor-like proteins, encoded by the genes knirps (kni) and tailless (tll), bind specifically to the element. In vitro, kni protein competes with the homeodomain-containing bcd protein in binding to a 16-base pair target sequence. In vivo experiments suggest that both kni and tll act as competitive repressors of bcd-mediated activation of Kr. These results suggest a mechanism by which developmental genes can be regulated in response to an activating morphogen gradient antagonized by repressors.