Structure-specific binding and photosensitized cleavage of branched DNA three-way junction complexes by cationic porphyrins.
Structure-specific binding and photosensitized cleavage of branched DNA three-way junction complexes by cationic porphyrins.
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阳离子卟啉对分支 DNA 三路连接复合物的结构特异性结合和光敏裂解。
DOI：
10.1111/j.1751-1097.1994.tb02977.x
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发表时间：
1994
影响因子：
3.3
通讯作者：
Leontis,NB
中科院分区：
文献类型：
--
作者：
Nussbaum,JM;Newport,ME;Mackie,M;Leontis,NB
使用天然凝胶电泳、吸收光谱和使用DNA测序技术的光化学探针研究了阳离子卟啉与形成分支的三向连接复合物（TWJ）的DNA寡核苷酸的相互作用。发现Meso-四（对-N-三甲基苯胺基）卟啉（TMAP）、meso-四（4-N-甲基吡啶基）卟啉（T4 MPyP）和meso-四（4-N-甲基吡啶基）卟啉（T3 MPyP）与DNA TWJ的结合比与DNA双链体的结合更紧密。在高离子强度下，与连接DNA的结合持续存在，这大大降低了卟啉与双链体DNA的结合亲和力。TMAP和T4 MPyP的TWJ DNA结合位点位于连接区，这是基于对连接区侧翼鸟苷残基的位点和结构特异性、卟啉敏化光损伤的观察。
The interactions of cationic porphyrins with DNA oligonucleotides that form branched, three‐way junction complexes (TWJ) were investigated using native gel electrophoresis, absorption spectroscopy and photochemical probing using DNA sequencing techniques. Meso‐tetra(pa ra‐N‐trimethylaniliniumyrjporphine (TMAP),meso‐tetra(4‐JV‐methylpyridiniumyl)porphine (T4MPyP) and meso‐tetra(4‐N‐methylpyridiniumytyporphine (T3MPyP) were found to bind more tightly to DNA TWJ than to DNA duplexes. The binding to the junction DNA persists at high ionic strength, conditions that greatly decrease porphyrin binding affinity to duplex DNA. The TWJ DNA binding sites of TMAP and T4MPyP were localized to the junction region based on the observation of site‐ and structure‐specific, porphyrin‐sensitized photodamage to guanosine residues flanking the junction region.