Myocardial antioxidant enzyme activities and concentration and glutathione metabolism in experimental hyperthyroidism
Myocardial antioxidant enzyme activities and concentration and glutathione metabolism in experimental hyperthyroidism
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DOI：
10.1016/j.mce.2006.02.005
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发表时间：
2006-04-25
影响因子：
4.1
通讯作者：
Belló-Klein, A
中科院分区：
文献类型：
--
作者：
Araujo, ASR;Ribeiro, MFM;Belló-Klein, A
L-甲状腺素给药（12 mg/L饮水，4周）诱导大鼠甲状腺功能亢进。对动物进行血流动力学评估，并进行心脏、肺和肝脏形态测定。测定心脏匀浆中的脂质过氧化（LPO）和蛋白质氧化（羰基）。western blot法测定小鼠谷胱甘肽（GSH）代谢、抗氧化酶活性及蛋白表达。治疗结束时，观察到心脏肥厚，左室收缩压和舒张末压升高，肺和肝充血。甲亢组LPO和羰基增加，第四周GSH下降46%。心肌氧化应激时间过程分析显示，治疗第2周心肌氧化应激增加。甲状腺机能亢进组抗氧化酶活性升高，第4周出现蛋白表达诱导。这些结果提示甲亢引起心肌功能障碍与氧化应激诱导抗氧化酶活性和蛋白表达有关。2006爱思唯尔爱尔兰有限公司版权所有。
Hyperthyroidism was induced in rats by L-thyroxine administration (12 mg/L in drinking water, 4 weeks). Animals were assessed hemodynamically, and heart, lung, and liver morphometry were performed. Lipid peroxidation (LPO) and protein oxidation (carbonyls) were measured in heart homogenates. It was quantified glutathione (GSH) metabolism, and antioxidant enzyme activities its and protein expression (by Westem blot). At the end of treatment, it was observed cardiac hypertrophy, elevation of left ventricular systolic and end diastolic pressures, lung and liver congestion. LPO and carbonyls were increased in the hyperthyroid group, and GSH was decreased by 46% in the fourth week. Myocardial oxidative stress time course analysis revealed that it was increased in the second week of treatment. Antioxidant enzyme activities elevation was accompanied by protein expression induction in the hyperthyroid group in the fourth week. These results imply that hyperthyroidism generates myocardial dysfunction associated with oxidative stress inducing antioxidant enzyme activities and protein expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.