Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
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发表时间：
1981-03
期刊：
影响因子：
11.2
通讯作者：
M. Rousset;A. Zweibaum;J. Fogh
中科院分区：
文献类型：
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作者：
M. Rousset;A. Zweibaum;J. Fogh
摘要 本研究的目的是调查糖原的异常积累及其与细胞生长过程的关系（已在人类结肠肿瘤细胞系中显示）是否也适用于源自其他组织的恶性细胞系。在 58 个人类肿瘤细胞系培养物的指数生长期期间测量了糖原含量，其中大多数来自糖原储存量较低的组织。糖原存在于所有细胞系中，其浓度因细胞系而异。在细胞系 Cama-1（乳腺）中发现了特别高的值，每毫克蛋白质含有 90 至 370 µg 糖原； A-498 和 SW-839（肾）； SK-MEL-24、MeWo 和 Malme-3M（黑色素瘤）； SK-UT-1（子宫）； VM-CUB-3 和 RT-4（膀胱）； U-87 MG（胶质母细胞瘤）；和 SK-OV-3（卵巢）。这些值高于在细胞系 SK-HEP-1（肝脏）和 JEG-3（绒毛膜癌）中发现的值，以及在 5 个子宫颈系中的 4 个（作为源自富含糖原的组织的对照肿瘤系）中发现的值。在源自黑色素瘤 (MeWo) 和肺癌 (SW-900)、卵巢癌 (SK-OV-3)、膀胱癌 (RT-4) 和肾癌 (SW-839) 的 5 种细胞系中研究了与细胞生长过程相关的糖原储存动力学。糖原含量的生长相关变化模式与结肠癌细胞中的变化模式相同。也就是说，在指数期的基础值达到稳定水平之后，糖原含量有规律地增加，从细胞分裂速率下降开始，当细胞生长停滞时达到最大值。在 SW-900、RT-4、SK-OV-3、MeWo 和 SW-839 细胞中发现的最大值分别为每毫克蛋白质 140 ± 5 (S.D.)、714 ± 42、804 ± 40、1070 ± 69 和 1206 ± 84 µg 糖原。这些细胞应该为进一步研究糖原代谢参与肿瘤过程和恶性细胞生长过程提供理想的材料。
Abstract The present study was undertaken to investigate whether the unusual accumulation of glycogen and its relationship to the process of cell growth, already shown in human colon tumor cell lines, also applied to malignant cell lines originating from other tissues. The glycogen content was measured during the exponential phase of growth in culture of 58 human tumor cell lines, most of them originating from tissues with low glycogen storage. Glycogen was present in all the cell lines at concentrations which varied from one cell line to another. Particularly high values, ranging from 90 to 370 µg of glycogen per mg of protein, were found in the cell lines Cama-1 (breast); A-498 and SW-839 (kidney); SK-MEL-24, MeWo, and Malme-3M (melanomas); SK-UT-1 (uterus); VM-CUB-3 and RT-4 (urinary bladder); U-87 MG (glioblastoma); and SK-OV-3 (ovary). These values were higher than those found in the cell lines SK-HEP-1 (liver) and JEG-3 (choriocarcinoma) and those found in 4 of 5 cervix lines taken as control tumor lines originating from glycogen-rich tissues. The kinetics of glycogen storage in relation to the process of cell growth was studied in 5 cell lines originating from a melanoma (MeWo) and from carcinomas of the lung (SW-900), ovary (SK-OV-3), urinary bladder (RT-4), and kidney (SW-839). The same pattern of growth-related variations of the glycogen content was found as that in colon carcinoma cells; namely, a plateau of basal value during the exponential phase was followed by a regular increase of the glycogen content, starting with the decrease in the rate of cell division and reaching maximum values when the cells came into arrest of growth. The maximum values found in SW-900, RT-4, SK-OV-3, MeWo, and SW-839 cells were, respectively, 140 ± 5 (S.D.), 714 ± 42, 804 ± 40, 1070 ± 69, and 1206 ± 84 µg of glycogen per mg of protein. These cells should provide ideal material for further studies on the involvement of glycogen metabolism in both the neoplastic process and the process of malignant cell growth.