Characterization and developmental expression of xSim, a Xenopus bHLH/PAS gene related to the Drosophila neurogenic master gene single-minded
Characterization and developmental expression of xSim, a Xenopus bHLH/PAS gene related to the Drosophila neurogenic master gene single-minded
复制标题
DOI：
10.1016/s0925-4773(00)00468-8
复制
发表时间：
2000-12-01
影响因子：
2.6
通讯作者：
Angelier, N
中科院分区：
文献类型：
--
作者：
Coumailleau, P;Penrad-Mobayed, M;Angelier, N
我们从非洲爪哇中分离到一个新的基因，命名为xSim，它编码一个bf 760个氨基酸的蛋白，含有一个基本的螺旋-环-螺旋(BHLH)基序，该基序与一个新的转录调控家族的PAS结构域相连，称为bHLH/PAS。XSim与果蝇单一基因(DSim)以及小鼠Sim1和Sim2蛋白有很强的氨基酸序列同源性。系统发育分析表明，xSim基因是mSim2基因的同源基因。时空分析表明，在非洲爪哇早期发育过程中，xSim以母体和合子的形式表达。原位杂交分析表明，转录本在动物大脑半球中丰富，直到囊胚期，并在原肠胚早期延伸到边缘地带。随着研究的深入，xSim主要局限于中枢神经系统。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
We have isolated a novel gene from Xenopus, denominated xSim, which encodes a protein bf 760 amino acids containing a basic helix-loop-helix (bHLH) motif contiguous to a PAS domain characteristic of an emerging family of transcriptional regulators so called bHLH/PAS. xSim shares a strong amino acid sequence identity with the Drosophila Single-minded (dSim) and with the murine Sim1 and Sim2 proteins. Phylogenetic analysis reveals that xSim gene is an ortholog gene of the mSim2 gene. Spatio-temporal analysis shows a maternal and a zygotic expression of xSim throughout early Xenopus development. In situ hybridization assays reveal that the transcripts are enriched in the animal hemisphere until blastula stage and extend to the marginal zone at early gastrula stage. As development proceeds, xSim is mainly restricted to the central nervous system. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.