Expression of small RNAs of Bordetella pertussis colonizing murine tracheas
Expression of small RNAs of Bordetella pertussis colonizing murine tracheas
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定植于小鼠气管的百日咳博德特氏菌小RNA的表达
DOI：
10.1111/1348-0421.12791
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发表时间：
2020
影响因子：
2.6
通讯作者：
Horiguchi Yasuhiko
中科院分区：
文献类型：
--
作者：
Hiramatsu Yukihiro;Suzuki Koichiro;Motooka Daisuke;Nakamura Shota;Horiguchi Yasuhiko
我们对百日咳病原体百日咳博德特拉进行了RNA测序，并鉴定出9个新的小RNA (sRNAs)，这些RNA在小鼠气管细菌定植过程中被转录。其中，有4种sRNAs在活体中比在体外表达更强烈。此外，8种sRNAs的表达不受BvgAS双组分系统的调控，而BvgAS是导致细菌感染的基因表达的主要调控因子。目前的研究结果表明，BvgAS独立的基因调控系统涉及在b过程中活跃的sRNAs。pertussisinfection。
We performed RNA sequencing onBordetella pertussis, the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressedin vivothanin vitro. Moreover, the expression of eight sRNAs was not regulated by the BvgAS two‐component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS‐independent gene regulatory system involving the sRNAs that is active duringB. pertussisinfection.