FleQ, a Transcriptional Activator, Is Required for Biofilm Formation In Vitro But Does Not Alter Virulence in a Cholesteatomas Model.
FleQ, a Transcriptional Activator, Is Required for Biofilm Formation In Vitro But Does Not Alter Virulence in a Cholesteatomas Model.
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FleQ 是一种转录激活剂，是体外生物膜形成所必需的，但不会改变胆脂瘤模型中的毒力。
DOI：
10.1097/mao.0000000000001088
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Chole,RichardA
中科院分区：
文献类型：
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作者：
Kao,WeeTinK;Gagnon,PatriciaM;Vogel,JosephP;Chole,RichardA
背景：感染性中耳炎比未感染的中耳炎更具破坏性，感染更持久。慢性和持久性的感染内中耳炎可能是因为存在的生物膜formation.Methods：27个突变株的PAO 1表面粘附进行了筛选。还筛选这些菌株的静态生物膜形成。通过用结晶紫染色来定量生物膜。然后通过耳道结扎在蒙古沙土鼠中诱导耳乳突瘤。在结扎时，耳道接种野生型PAO 1和铜绿假单胞菌的生物膜缺陷型PAO 1 ΔfleQ菌株。在术后第7天开始环丙沙星（20 mg/kg/天）的7周疗程。结果：PAO 1 ΔfleQ是一株粘附性差、生物膜形成缺陷的铜绿假单胞菌突变株。感染性中耳炎较未感染的中耳炎有更多的生长、骨破坏和骨重建。结论：生物膜表型不是铜绿假单胞菌感染中耳瘤的重要毒力因子。
Background:Infected aural cholesteatomas have been demonstrated to be more destructive than uninfected cholesteatomas and infections are more persistent. The chronicity and persistence of infections within cholesteatomas may be because of the presence of biofilm formation.Methods:Twenty-seven mutant strains of PAO1 were screened for surface adherence. These strains were also screened for static biofilm formation. The biofilms were quantified by staining with crystal violet. Aural cholesteatomas were then induced in Mongolian gerbils by ligation of the ear canal. At the time of ligation, the ear canals were inoculated with wild-type PAO1 and a biofilm deficient PAO1 ΔfleQ strain of P. aeruginosa. A 7 weeks course of ciprofloxacin (20 mg/kg/day) was started on postoperative day 7. Eight weeks after induction of cholesteatomas, the cholesteatoma size, levels of bone destruction, and levels of bone remodeling were evaluated using microCT imaging.Results:PAO1 ΔfleQ was identified as a poorly adherent and deficient biofilm forming mutant strain of P. aeruginosa. Infected cholesteatomas had more growth, bone destruction and bone remodeling than uninfected cholesteatomas. However, there was no difference observed between cholesteatomas infected with PAO1 (biofilm competent strain) and PAO1 ΔfleQ (biofilm deficient strain).Conclusion:We demonstrate that the biofilm phenotype is not an important virulence factor in cholesteatomas infected with P. aeruginosa.