Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice
Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice
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DOI：
10.1111/j.1399-6576.2012.02691.x
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发表时间：
2012-09-01
影响因子：
2.1
通讯作者：
Ma, D.
中科院分区：
文献类型：
--
作者：
Fidalgo, A. R.;Cibelli, M.;Ma, D.
本研究的目的是研究异氟醚和丁丙诺啡诱导的全身麻醉对小鼠海马依赖性记忆和新皮层依赖性记忆的影响，此外，比较这种麻醉对这些记忆过程的影响与脂多糖（LPS）给药对相同记忆过程的影响。方法采用异氟醚（15？min），或LPS 100 μ g/kg（腹腔注射）24？在恐惧条件反射之前或之后。在第4天，使用情境恐惧条件反射任务评估这些治疗对大脑皮层依赖性记忆的影响。为了评估新皮质依赖的记忆，异氟烷麻醉或LPS给予72？在情境恐惧条件反射后h。在第32天进行新皮质依赖性记忆评估。结果与LPS注射不同，异氟醚和丁丙诺啡诱导的麻醉不会损害大脑皮层依赖的恐惧条件记忆任务中的冻结反应。顺行性遗忘评估：麻醉组为49.67 +/- 6.87%，对照组为54.5 +/- 4.12%。逆行性遗忘评估：麻醉组为47.16 ± 8.71%，对照组为54.5 ± 4.12%; P <0.05。0.05.因此，异氟烷和丁丙诺啡都不会损害海马依赖性记忆。然而，在新皮层依赖的记忆任务中，异氟醚诱导的麻醉和LPS诱导的炎症均导致冻结反应减少：麻醉组为62.13 +/- 5.80%，LPS组为74.63 +/- 5.69%，对照组为81.75 +/- 3.26%; P？
Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus-dependent and neocortex-dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the effects induced by lipopolysaccharide (LPS) administration on the same memory processes. Methods To assess hippocampus-dependent memory, isoflurane (for 15?min) after buprenorphine injection, or LPS 100 mu g/kg (intraperitoneally) was administered 24?h before or after fear conditioning. The effect of these treatments on hippocampus-dependent memory was assessed using contextual fear-conditioning tasks at day 4. To assess neocortex-dependent memory, isoflurane anaesthesia or LPS was given 72?h after contextual fear conditioning. Neocortex-dependent memory assessment was performed at day 32. Results Unlike LPS injection, isoflurane with buprenorphine-induced anaesthesia does not impair freezing responses in hippocampus-dependent fear-conditioning memory tasks. On anterograde amnesia assessment: 49.67 +/- 6.87% for the anaesthesia group and 54.5 +/- 4.12% for the control group. On retrograde amnesia assessment: 47.16 +/- 8.71% for the anaesthesia group and 54.5 +/- 4.12% for control group; P?>?0.05. Thus, neither isoflurane nor buprenorphine impair hippocampus-dependent memory. However, on the neocortex-dependent memory task, both isoflurane-induced anaesthesia and LPS-induced inflammation result in reduced freezing responses: 62.13 +/- 5.80% for the anaesthesia group, 74.63 +/- 5.69% for the LPS group, and 81.75 +/- 3.26% for the control group; P?