Pathophysiologic correlates of acute porcine pleuropneumonia
Pathophysiologic correlates of acute porcine pleuropneumonia
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DOI：
10.2460/ajvr.2000.61.684
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发表时间：
2000-06-01
影响因子：
1
通讯作者：
Murtaugh, MP
中科院分区：
文献类型：
--
作者：
Baarsch, MJ;Foss, DL;Murtaugh, MP
目的-开发和评估体内模型以研究急性猪胸膜肺炎发病机制的早期事件。动物-36头6至8周龄的猪。程序-猪鼻内或气管内接种胸膜肺炎放线杆菌；通过评估临床症状、肉眼和显微镜下肺部病变、血液学变化、血清锌、铁和触珠蛋白浓度以及炎性细胞因子来比较接种途径。结果-2种接种途径产生相似的结果，尽管鼻内接种引起单侧肉眼肺部病变，而气管内接种引起双侧肉眼肺部病变。 气管内接种后 2 小时内和鼻内接种后 6 至 8 小时内观察到疾病的临床症状。通过任一接种途径接种后，白细胞总数没有显着差异，但带状中性粒细胞显着增加。与胸膜肺炎杆菌接种相关的最早发现，无论途径如何，血清锌和铁浓度降低。接种后血清触珠蛋白浓度显着升高。接种诱导巨噬细胞快速流入肺部并局部诱导促炎细胞因子。对肺组织总 RNA 的 Northern 印迹分析表明，接种猪的白细胞介素 (IL)-1 β、IL-1 α 和 IL-8 浓度增加；肿瘤坏死因子信使RNA浓度未升高。结论-气管内接种胸膜肺炎A型胸膜肺炎可快速、持续诱导弥漫性双侧肺炎；因此，该方法可用于研究与细菌性肺炎相关的急性病理生理变化，并可为测试预防和治疗这种猪呼吸道疾病和其他呼吸道疾病的方法提供实验模型。
Objective-To develop and evaluate an in vivo model to study early events in the pathogenesis of acute porcine pleuropneumonia.Animals-Thirty-six 6- to 8-week-old pigs.Procedure-Pigs were inoculated intranasally or endotracheally with Actinobacillus pleuropneumoniae; inoculation routes were compared by evaluation of clinical signs, gross and microscopic lung lesions, hematologic changes, serum zinc, iron, and haptoglobin concentrations, and inflammatory cytokines.Results-The 2 inoculation routes resulted in similar findings, although intranasal inoculation caused unilateral gross lung lesions, whereas endotracheal inoculation caused bilateral gross lesions, Clinical signs of disease were observed < 2 hours after endotracheal inoculation and 6 to 8 hours after intranasal inocula- tion. Total WBC counts did not differ significantly after inoculation by either inoculation route, although band neutrophils increased significantly. The earliest findings associated with A pleuropneumoniae inoculation, irrespective of route, were decreased serum zinc and iron concentrations. Serum haptoglobin concentrations were significantly increased after inoculation. inoculation induced rapid influx of macrophages into the lung and local induction of proinflammatory cytokines. Northern blot analysis of total RNA from lung tissue indicated that inoculated pigs had increased concentrations of interleukin (IL)-1 beta, IL-1 alpha, and IL-8; tumor necrosis factor messenger RNA concentration was not increased.Conclusions-Endotracheal inoculation with A pleuropneumoniae rapidly and consistently induced diffuse bilateral pneumonia; thus, this method may be useful for the study of acute pathophysiologic changes associated with bacterial pneumonia and may provide an experimental model for testing modalities for prevention and treatment of this and other respiratory tract diseases of pigs.