Comparative study of collagen distribution in the dermis of the embryonic carapace of soft- and hard-shelled cryptodiran turtles
Comparative study of collagen distribution in the dermis of the embryonic carapace of soft- and hard-shelled cryptodiran turtles
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软、硬甲龟胚胎甲壳真皮中胶原蛋白分布的比较研究
DOI：
10.1002/jmor.21327
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发表时间：
2021
影响因子：
1.5
通讯作者：
Qian Guoying
中科院分区：
文献类型：
--
作者：
Yang Jie;Song Wei;Li Caiyan;Fang Chanlin;Zhang Yuting;Wang Qingqing;Zhang Mingxing;Qian Guoying
海龟的特征是其典型的甲壳，主要由角质部分的角质β蛋白和真皮部分的胶原蛋白组成。比较了硬壳龟和软壳龟背甲真皮中细胞外基质的形成。本研究采用比较形态学和胚胎学分析方法，研究了软壳龟Pelodiscus sinensis和硬壳龟Trachemys scripta elegans的甲壳发育，重点是胶原蛋白的积累。组织学结果表明，随着胚胎的发育，龟背甲中胶原沉积逐渐增多。然而，显着的差异，观察两种海龟在发育阶段的检查。本文报道了中国对虾背甲真皮的显微结构。中华绒螯蟹的甲壳基质中胶原纤维束呈明暗相间的带状，胶原含量较高; scripta的特点是松散的和较薄的胶原纤维束，I型胶原的百分比较低。总的来说，在特定的发育阶段，胶原纤维的形成和分布在软壳龟和硬壳龟之间是不同的。这些结果表明，软壳龟柔韧的表皮由规则分布有胶原蛋白的坚固真皮支撑，并且它允许改善操纵，而在硬壳龟的情况下观察到的坚固但不灵活的表皮限制了运动。
Turtles are characterized by their typical carapace, which is primarily composed of corneous beta proteins in the horny part and collagen in the dermal part. The formation of the extracellular matrix in the dermis of the carapace in a hard‐shelled and a soft‐shelled turtle has been compared. The study examines carapace development, with an emphasis on collagen accumulation, in the soft‐shelled turtlePelodiscus sinensisand hard‐shelled turtleTrachemys scripta elegans, using comparative morphological and embryological analyses. The histological results showed that collagen deposition in the turtle carapace increased as the embryos developed. However, significant differences were observed between the two turtle species at the developmental stages examined. The microstructure of the dermis of the carapace ofP. sinensisshowed light and dark banding of collagen bundles, with a higher overall collagen content, whereas the carapacial matrix ofT. scriptawas characterized by loosely packed and thinner collagenous fiber bundles with a lower percentage of type I collagen. Overall, the formation and distribution of collagen fibrils at specific developmental stages are different between the soft‐and hard‐shelled turtles. These results indicate that the pliable epidermis of the soft‐shelled turtle is supported by a strong dermis that is regularly distributed with collagen and that it allows improved maneuvering, whereas a strong but inflexible epidermis as observed in case of hard‐shelled turtles limits movement.