Pemphigus foliaceus antibodies and a monoclonal antibody to desmoglein I demonstrate stratified squamous epithelial-specific epitopes of desmosomes.
Pemphigus foliaceus antibodies and a monoclonal antibody to desmoglein I demonstrate stratified squamous epithelial-specific epitopes of desmosomes.
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落叶型天疱疮抗体和桥粒芯糖蛋白 I 单克隆抗体证明了桥粒的分层鳞状上皮特异性表位。
DOI：
10.1097/00000372-198712000-00007
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
J. Stanley
中科院分区：
文献类型：
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作者：
N. Rubinstein;J. Stanley
落叶型天疱疮（PF）是一种自身免疫性疾病，其中自身抗体针对表皮细胞的细胞表面。最近，我们使用免疫印迹技术证明了一个亚组的PF患者有自身抗体的桥粒核心糖蛋白，桥粒芯糖蛋白I（DGI）。桥粒存在于所有上皮细胞和心脏中，但PF仅影响复层鳞状上皮细胞。这一发现的一种解释可能是桥粒存在组织特异性差异。因此，为了确定DGI的某些表位是否是组织限制性的，我们用以下抗体对各种猴组织进行了免疫荧光研究：针对整个桥粒的兔多克隆抗血清，其证明了所有测试组织中的桥粒;针对DGI的小鼠单克隆抗体MmDGI-1;以及在免疫印迹上结合DGI的PF血清（PF IB+）。此外，我们通过免疫印迹测试了具有不结合DGI的PF血清（PF IB-）的组织，以确定这些血清在其组织特异性方面是否不同于PF IB+血清。PF IB+、PF IB-和MmDGI-1抗体染色所有检测的分层鳞状上皮，包括皮肤、舌、食管上段、结膜和角膜;然而，它们不染色心脏或任何非分层鳞状上皮，包括胆囊、小肠、肝脏、输尿管和膀胱。这些结果表明，有组织异质性的桥粒，和PF抗体和单克隆抗体定义的DGI上的表位只存在于复层鳞状上皮。此外，PF IB-血清与PF IB+血清具有相同的组织特异性.这些结果可能部分解释了为什么PF只涉及复层鳞状上皮。
Pemphigus foliaceus (PF) is a human autoimmune disease in which autoantibodies are directed against the cell surface of epidermal cells. Using an immunoblotting technique, we recently demonstrated that a subgroup of PF patients have autoantibodies to the desmosomal core glycoprotein, desmoglein I (DGI). There are desmosomes in all epithelia and in heart, yet PF affects only stratified squamous epithelia. One explanation for this finding might be that there are tissue-specific differences in desmosomes. Thus, to determine whether certain epitopes of DGI are tissue-restricted, we performed immunofluorescence studies on various monkey tissues with the following antibodies: a rabbit polyclonal antiserum against whole desmosomes, which demonstrated the desmosomes in all tissues tested; a mouse monoclonal antibody against DGI, MmDGI-1; and PF sera that bound DGI on immunoblotting (PF IB+). In addition, we tested the tissues with PF sera that did not bind DGI by immunoblotting (PF IB-) to determine if these sera were different from PF IB+ sera in their tissue specificity. PF IB+, PF IB-, and MmDGI-1 antibodies stained all stratified squamous epithelia tested, including skin, tongue, upper esophagus, conjunctiva, and cornea; however, they did not stain heart or any nonstratified squamous epithelia, including gall bladder, small intestine, liver, ureter, and bladder. These results indicate that there is tissue heterogeneity of desmosomes, and that epitopes on DGI defined by both PF antibodies and a monoclonal antibody are present only in stratified squamous epithelia. In addition, PF IB- sera had the same tissue specificity as PF IB+ sera. These results may partially explain why PF involves only stratified squamous epithelia.