Chitooligosaccharides -: preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity
Chitooligosaccharides -: preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity
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DOI：
10.1016/j.carres.2005.02.005
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发表时间：
2005-05-02
影响因子：
3.1
通讯作者：
Tharanathan, RN
中科院分区：
文献类型：
--
作者：
Kittur, FS;Kumar, ABV;Tharanathan, RN
黑曲霉果胶酶同工酶具有多聚半乳糖醛酸酶活性，在pH值为3.5的条件下产生壳聚糖(86%)、壳寡糖(4.8%)和单体(2.2%)。木炭-硅藻土层析和再N-乙酰化反应以及CD、IR、MALDI-TOF-MS和FAB-MS分析表明，CoS中含有丰富的甲壳二糖、甲壳三糖和甲壳四糖。与天然壳聚糖相比，CoS-单体混合物对蜡状芽孢杆菌和大肠杆菌的杀灭效果更好。在壳寡聚体中，六聚体的抑菌效果最好，其次是五聚体、四聚体、三聚体和二聚体。在两种单体中，只有GlcN有轻微的细菌生长抑制作用。扫描电子显微镜显示CoS-单体混合物对蜡样芽孢杆菌和大肠杆菌的杀菌作用模式。(C)2005爱思唯尔有限公司。保留所有权利。
An isozyme of pectinase from Aspergillus niger with polygalacturonase activity caused chitosanolysis at pH 3.5, resulting in low-molecular weight chitosan (86%), chitooligosaccharides (COs, 4.8%) and monomers (2.2%). HPLC showed the presence of COs with DP ranging from 2 to 6. Charcoal-Celite chromatography and re-N-acetylation of the COs followed by CD, IR, MALDI-TOF-MS and FAB-MS analyses revealed an abundance of chitobiose, chitotriose and chitotetraose. The COs-monomeric mixture showed a bactericidal effect towards Bacillus cereus and Escherichia coli more efficiently than native chitosan. Among the chitooligomers, the hexamer showed maximum antibacterial effect followed by the penta-, tetra-, tri- and dimers. Of the two monomers, only GlcN showed slight bacterial growth inhibition. SEM revealed bactericidal action patterns of COs-monomeric mixture towards B. cereus and E. coli. (c) 2005 Elsevier Ltd. All rights reserved.