Fasting concentrations of nesfatin-1 are negatively correlated with body mass index in non-obese males
Fasting concentrations of nesfatin-1 are negatively correlated with body mass index in non-obese males
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DOI：
10.1111/j.1365-2265.2010.03835.x
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发表时间：
2010-10-01
影响因子：
3.2
通讯作者：
Mori, Masatomo
中科院分区：
文献类型：
--
作者：
Tsuchiya, Takafumi;Shimizu, Hiroyuki;Mori, Masatomo
背景我们最近发现了一种新的促凋亡蛋白nesfatin-1，它是由nesfatin/nucleobindin-2（NUCB-2）加工而成的。目的为了探讨nesfatin-1在人体中的临床意义，我们建立了一种新的检测人外周血nesfatin-1的特异性酶联免疫吸附试验（ELISA），并测定了健康人外周血中nesfatin-1的循环浓度。在空腹过夜的条件下，随后进行口服葡萄糖耐量和膳食试验。（年龄：24中心点5 +/-0中心点6，体重指数（BMI）; 21个中心点1 +/-0中心点3 kg/m2）被招募到研究中以评估奈法汀-1的空腹浓度。其中，15名受试者接受了75克口服葡萄糖耐量试验（OGTT），另外15名受试者接受了进餐试验。此外，对9例高BMI男性（年龄：32中心点4 +/-3中心点7，BMI; 37中心点3 +/-3中心点8 kg/m2）空腹血清nesfatin-1浓度进行测定。在OGTT和膳食试验期间，Nesfatin-1浓度没有显著变化。高BMI组空腹nesfatin-1水平显著低于非肥胖组（P <0.05）。进一步积累临床观察是必要的，以澄清循环nesfatin-1在各种代谢紊乱中的作用。
P>BackgroundWe recently identified a novel anorexigenic protein, nesfatin-1, which is processed from nesfatin/nucleobindin-2 (NUCB2). However, the clinical importance of this protein has not been determined.ObjectiveTo investigate its clinical significance in humans, we have established a new specific enzyme-linked immunosorbent assay (ELISA) for human nesfatin-1 in peripheral blood and measured its circulating concentration in healthy subjects.DesignThe new sandwich-type ELISA method was validated and then used to measure nesfatin-1 levels in plasma samples, under overnight fasting conditions, followed by oral glucose tolerance and meal tests.Patients and measurementsA total of 43 nonobese males (age: 24 center dot 5 +/- 0 center dot 6 , body mass index (BMI); 21 center dot 1 +/- 0 center dot 3 kg/m2) were recruited to the study for evaluating fasting concentrations of nesfatin-1. In those, fifteen subjects underwent a 75- g oral glucose tolerance test (OGTT) and another 15 underwent a meal test. In addition, fasting concentrations of nesfatin-1 were measured in nine males with high BMI (age: 32 center dot 4 +/- 3 center dot 7 , BMI; 37 center dot 3 +/- 3 center dot 8 kg/m2).ResultsPeripheral concentrations of nesfatin-1 showed a significant negative correlation with BMI, percentage body fat, body fat weight and blood glucose (P < 0 center dot 05). Nesfatin-1 concentrations were not significantly changed during OGTT and meal tests. Fasting nesfatin-1 levels were significantly lower in subjects with high BMI compared to nonobese subjects (P < 0 center dot 05).ConclusionsA new specific and sensitive ELISA for nesfatin-1 was established. Further accumulation of clinical observations is necessary to clarify the role of circulating nesfatin-1 in various metabolic disorders.