The ankyrin repeat domain of Huntingtin interacting protein 14 contains a surface aromatic cage, a potential site for methyl-lysine binding.

The ankyrin repeat domain of Huntingtin interacting protein 14 contains a surface aromatic cage, a potential site for methyl-lysine binding.
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DOI:
10.1002/prot.22452
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发表时间:
2009-08-15
影响因子:
2.9
通讯作者:
Cheng, Xiaodong
Cheng, Xiaodong
中科院分区:
生物学4区
文献类型:
--
作者:
Gao, Tiyu;Collins, Robert E.;Horton, John R.;Zhang, Xing;Zhang, Rongguang;Dhayalan, Arunkumar;Tamas, Raluca;Jeltsch, Albert;Cheng, Xiaodong

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从ATCC克隆5266097中扩增编码人HIP 14的N-末端51-288残基的基因片段(我们称之为HIP 14 ANK),并将其克隆到含有6xHis标签和SUMO标签的修饰的pET 28 b载体的NdeI和XhoI限制性位点中,产生pXC 650。
Materials and MethodsThe gene fragment encoding the N-terminal 51-288 residues of human HIP14 (we termed it the HIP14ANK) was amplified from ATCC Clone 5266097 and cloned into the NdeI and XhoI restriction sites of a modified pET28b vector containing 6xHis tag followed by SUMO tag, yielding pXC650.
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