Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1
Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1
复制标题
胞浆内单精子注射后鹌鹑卵母细胞的受精和发育1
作者：
A. Hrabia;S. Takagi;T. Ono;K. Shimada
摘要本研究旨在建立鹌鹑体外受精和发育的卵胞浆内单精子注射（ICSI）方法。比较了1）自发排卵和过早排卵之间以及2）睾丸圆形精子细胞、细长精子细胞、未成熟精子和成熟精子之间卵母细胞的受精效率。将单个精子或精子细胞注入卵母细胞，培养24 h。用苏木精-伊红（HE）和核特异性荧光染料（DAPI）对细胞分裂进行组织学观察。自然排卵组和诱导排卵组分别有5/30（16.6%）和4/30（13.3%）的卵母细胞与成熟精子受精。这些胚胎在II-VII阶段发育。注射睾丸精子的卵母细胞数量的一半（6个中的3个）受精并发育到IV-VII期，注射细长精子细胞的5个卵母细胞中的2个受精并发育到VI期。所有注入圆形精子细胞的卵母细胞均未受精。结果表明，卵母细胞胞质内注射单个精子可激活卵母细胞并导致受精。与自发排卵的卵母细胞一样，过早排卵的卵母细胞也能与成熟精子结合。此外，结果表明，睾丸圆形精子细胞可能不具有足够的卵母细胞激活能力，但细长精子细胞和未成熟精子有能力参与受精和早期胚胎发育。
Abstract The present study was conducted to establish the intracytoplasmic sperm injection (ICSI) method for in vitro fertilization and development in quail. The efficiency of fertilization of oocytes was compared 1) between spontaneous and premature ovulation and 2) among testicular round spermatids, elongated spermatids, and immature and mature spermatozoa. The oocytes were injected with a single spermatozoon or spermatid and cultured for 24 h. Cell division was histologically observed with hematoxylin-eosin (HE) and a nucleus-specific fluorescent dye (DAPI). Five of 30 (16.6%) and 4 of 30 (13.3%) oocytes injected with mature sperm were fertilized in the spontaneous and induced ovulation group, respectively. Those embryos showed development at stages II–VII. Half the number (three of six) of the oocytes injected with testicular spermatozoa were fertilized and developed to stages IV–VII, and two of five oocytes injected with elongated spermatids were fertilized and developed to stage VI. All ooocytes injected with round spermatids were unfertilized. The results demonstrate that intracytoplasmic injection of a single sperm into quail oocyte can activate the oocyte and lead to fertilization. Oocytes prematurely ovulated are capable of fertilizing with mature sperm as are those spontaneously ovulated. In addition, the results suggest that the testicular round spermatids may not possess sufficient oocyte-activating potency but that the elongated spermatids and immature spermatozoa are competent to participate in fertilization and early embryonic development in quail.
影响因子：
4.6
作者：
Y. Kimura;Ryuzo Yanagimachi
通讯作者：
Y. Kimura;Ryuzo Yanagimachi
通过胞浆内注射睾丸精子和细长的精子细胞产生恒河猴后代。
影响因子：
6.7
作者：
Hewitson,Laura;Martinovich,Crista;Simerly,Calvin;Takahashi,Diana;Schatten,Gerald
通讯作者：
Schatten,Gerald