Transient receptor potential vanilloid 1-mediated expression and secretion of endothelial cell-derived calcitonin gene-related peptide
Transient receptor potential vanilloid 1-mediated expression and secretion of endothelial cell-derived calcitonin gene-related peptide
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瞬时受体电位香草酸1介导的内皮细胞源性降钙素基因相关肽的表达和分泌
DOI：
10.1016/j.regpep.2008.05.007
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发表时间：
2008-10-09
影响因子：
--
通讯作者：
Li, Yuan-Jian
中科院分区：
文献类型：
--
作者：
Luo, Dan;Zhang, Yi-Wei;Li, Yuan-Jian
降钙素基因相关肽（CGRP）是感觉神经中的主要递质，也可以在血管内皮中表达。瞬时受体电位香草酸 1 (TRPV1) 调节感觉神经中 CGRP 的合成和释放，也存在于内皮细胞中。本研究测试TRPV1是否调节内皮细胞CGRP的释放和合成，并评估内皮细胞来源的CGRP的保护作用。用辣椒素或热疗处理人脐静脉内皮细胞（HUVEC）。 RT-PCR检测CGRP mRNA水平，放射免疫法检测蛋白水平。溶血磷脂酰胆碱诱导内皮细胞损伤，并通过细胞活力和乳酸脱氢酶活性进行评估。 HUVEC 表达 CGRP，包括 α 和 β 亚型。辣椒素增加了培养基中CGRP的水平，并上调了内皮细胞中CGRP的表达。高温还增加了 CGRP mRNA 的水平。 TRPV1 的竞争性拮抗剂辣椒西平消除了这些作用。辣椒素显着减轻LPC诱导的内皮细胞损伤，而辣椒西平或CGRP受体拮抗剂CGRP(8-37)可预防和加重这种损伤。这些结果表明TRPV1还调节内皮细胞源性CGRP的表达和分泌，从而对内皮细胞产生保护作用。 (C) 2008 Elsevier B.V. 保留所有权利。
Calcitonin gene-related peptide (CGRP), the principal transmitter in sensory nerves, could also be expressed in vascular endothelium. Transient receptor potential vanilloid 1(TRPV1), which modulates the synthesis and release of CGRP in sensory nerves, is also present in endothelial cells. The present study tested whether TRPV1 modulates the release and synthesis of CGRP in endothelial cells, and evaluated the protective effect of endothelial cell-derived CGRP. Human umbilical vein endothelial cells (HUVECs) were treated with capsaicin or hyperthermia. The level of CGRP mRNA was detected by RT-PCR, and protein level was measured by radioimmunoassay. endothelial cell injury was induced by lysophosphatidylcholine, and evaluated by cell viability and lactate dehydrogenase activity. HUVECs expressed CGRP, both alpha- and beta-subtype. Capsaicin increased the level of CGRP in the culture medium, and up-regulated the expression of CGRP in endothelial cells. Hyperthermia also increased the level of CGRP mRNA. These effects were abolished by capsazepine, a competitive antagonist of TRPV1. Capsaicin significantly attenuated the endothelial cell damage induced by LPC, which was prevented and aggravated by capsazepine or CGRP(8-37), antagonist of CGRP receptor. These results indicate that TRPV1 also regulates the expression and secretion of endothelial cell-derived CGRP, which affords protective effects on endothelial cells. (C) 2008 Elsevier B.V. All rights reserved.