Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.
Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.
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通过脉冲场凝胶电泳技术分离白色念珠菌染色体大小 DNA 的比较。
DOI：
10.1093/nar/17.10.3783
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发表时间：
1989
影响因子：
14.9
通讯作者：
Medoff,G
中科院分区：
文献类型：
--
作者：
Lasker,BA;Carle,GF;Kobayashi,GS;Medoff,G
采用脉冲场凝胶电泳技术研究了c染色体大小的DNA分子。白色的。用正交场交替凝胶电泳（OFAGE）技术将两株白色念珠菌的染色体大小DNA分离成8个条带。在c染色体制备中观察到6条条带。使用场反转凝胶电泳（FIGE）。菌株间电泳迁移率的差异。白垩白检查表明，染色体长度多态性的存在，使其难以关联的带状模式之间的菌株。然而，通过分配c，这些相关性得到了促进。利用克隆的DNA探针对8个观察到的条带进行杂交。Southern blotting显示，6条FIGE条带由4条单线和2条配对双线组成，占OFAGE分析观察到的8条条带。OFAGE和FIGE分析的一致性表明，c。白双倍体基因组至少包含8条染色体。
Pulsed-field gel electrophoresis techniques were used to study chromosome-sized DNA molecules ofC. albicans. Chromosome-sized DNA of two strains ofCandida albicanshas been resolved into 8 bands by orthogonal-field-alternation gel electrophoresis (OFAGE). Six bands were observed in chromosomal preparations ofC. albicansusing field-inversion gel electrophoresis (FIGE). Differences in the electrophoretic mobilities of bands of the strains ofC. albicansexamined suggests that chromosome-length polymorphisms exist and make it difficult to correlate the banding patterns among strains. These correlations were facilitated, however, by assignment ofC. albicanschromosomes by hybridization using a collection of cloned DNA probes specific for each of the 8 observed bands. Southern blotting showed that the 6 FIGE bands consisted of 4 singlets and 2 comigrating doublets, accounting for the 8 bands observed by OFAGE analysis. The agreement between OFAGE and FIGE analysis suggests that theC. albicanshaploid genome contains a minimum of 8 chromosomes.