Phenylpropanoids and neolignans isolated from Myristica fragrans enhance glucose uptake in myotubes
Phenylpropanoids and neolignans isolated from Myristica fragrans enhance glucose uptake in myotubes
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从肉豆蔻中分离出的苯丙素和新木脂素可增强肌管中的葡萄糖摄取
DOI：
10.1039/d1fo04408g
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Ashida Hitoshi
中科院分区：
文献类型：
--
作者：
Yoshioka Yasukiyo;Kono Ryunoshin;Kuse Masaki;Yamashita Yoko;Ashida Hitoshi
肉豆蔻是肉豆蔻或其粉末的种子，用作香料和传统药物。肉豆蔻的抗糖尿病作用目前还不完全清楚。在这项研究中，我们研究了香肉豆蔻中与葡萄糖摄取有关的活性化合物的分离和鉴定，并阐明了它们在L6肌管中的作用机制。分离鉴定了肉豆蔻素、甘草素B、阿多糖苷(erythro-2-(4-allyl-2，6-dimethoxy-phenoxy)-1-(3，4-dimethoxyphenyl)-propan-1-ol)和阿多糖苷((7S，8R)-2-(4-allyl-2，6-dimethoxyphenoxy)-1-(3，4，5-trimethoxyphenyl)-propan-1-ol)。肉豆蔻素或ADDP和ADTP的混合物在处理15min后通过AMP活化蛋白激酶的磷酸化促进葡萄糖转运蛋白4(GLUT4)在L6肌管中的转位，而甘草素B在处理后240min促进葡萄糖转运蛋白4(GLUT4)的易位。口服含有这些活性化合物的肉豆蔻花提取物给ICR小鼠，可抑制餐后高血糖。因此，肉豆蔻是一种很有前途的功能食品，可以通过促进肌肉对葡萄糖的摄取来预防餐后高血糖和2型糖尿病。
Nutmeg is the seed of Myristica fragrans or its powder and is used as a spice and a traditional medicine. The antidiabetic effect of nutmeg is not fully understood yet. In this study, we examine the isolation and identification of the active compounds of Myristica fragrans with regards to glucose uptake and elucidate their mechanism in L6 myotubes. Myrisiticin, licarin B, erythro-2-(4-allyl-2,6-dimethoxy-phenoxy)-1-(3,4-dimethoxyphenyl)-propan-1-ol (ADDP) and (7S,8R)-2-(4-allyl-2,6-dimethoxyphenoxy)-1-(3,4,5-trimethoxyphenyl)-propan-1-ol (ADTP) were isolated and identified as the active compounds. Myristicin or a mixture of ADDP and ADTP promoted the translocation of glucose transporter 4 (GLUT4) through phosphorylation of AMP-activated protein kinase in L6 myotubes 15 min after treatment, while licarin B promoted it 240 min after treatment. Oral administration of the fraction from Myristica fragrans containing these active compounds to ICR mice suppressed post-prandial hyperglycemia. Thus, Myristica fragrans is a promising functional food to prevent post-prandial hyperglycemia and type 2 diabetes mellitus by promoting glucose uptake in muscle.