The cag PAI is intact and functional but HP0521 varies significantly in Helicobacter pylori isolates from Malaysia and Singapore
The cag PAI is intact and functional but HP0521 varies significantly in Helicobacter pylori isolates from Malaysia and Singapore
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DOI：
10.1007/s10096-010-0881-7
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发表时间：
2010-04-01
影响因子：
4.5
通讯作者：
Mitchell, H.
中科院分区：
文献类型：
--
作者：
Schmidt, H-M. A.;Andres, S.;Mitchell, H.
幽门螺杆菌相关疾病至少部分归因于感染菌株的基因型，特别是特异性毒力因子的存在。我们调查了一种新的H. pylori毒力因子，包括cag致病岛（派），以及它们与马来西亚和新加坡三个主要种族分离株中严重疾病的相关性，并评估cag派是否完整和体外功能。用聚合酶链反应（PCR）检测159例HLA-DUPA、cagA、cagE、cagT、cagL和babA基因，并对vacA、EPIYA基序、HP 0521等位基因和oipA ON状态进行分型。幽门螺杆菌临床分离株。在体外对22株菌株进行了IL-8诱导和CagA易位的研究。cagA、cagE、cagL、cagT、babA、oipA ON和vacA s1和i1的患病率> 85%，与疾病状态或种族无关。dupA和主要的HP 0521等位基因和EPIYA基序的患病率随种族而显著变化（p < 0.05）。一个完整的cag派的高患病率被发现在所有的种族群体，但是，没有任何毒力因子和疾病状态之间的关联。HP 0521等位基因、EPIYA基序和宿主种族之间的新关联表明，进一步研究以确定该基因的功能是重要的。
Helicobacter pylori-related disease is at least partially attributable to the genotype of the infecting strain, particularly the presence of specific virulence factors. We investigated the prevalence of a novel combination of H. pylori virulence factors, including the cag pathogenicity island (PAI), and their association with severe disease in isolates from the three major ethnicities in Malaysia and Singapore, and evaluated whether the cag PAI was intact and functional in vitro. Polymerase chain reaction (PCR) was used to detect dupA, cagA, cagE, cagT, cagL and babA, and to type vacA, the EPIYA motifs, HP0521 alleles and oipA ON status in 159 H. pylori clinical isolates. Twenty-two strains were investigated for IL-8 induction and CagA translocation in vitro. The prevalence of cagA, cagE, cagL, cagT, babA, oipA ON and vacA s1 and i1 was > 85%, irrespective of the disease state or ethnicity. The prevalence of dupA and the predominant HP0521 allele and EPIYA motif varied significantly with ethnicity (p < 0.05). A high prevalence of an intact cag PAI was found in all ethnic groups; however, no association was observed between any virulence factor and disease state. The novel association between the HP0521 alleles, EPIYA motifs and host ethnicity indicates that further studies to determine the function of this gene are important.