Gene expression profiling of the developing Drosophila CNS midline cells
Gene expression profiling of the developing Drosophila CNS midline cells
复制标题
DOI：
10.1016/j.ydbio.2004.08.047
复制
发表时间：
2004-11-15
影响因子：
2.7
通讯作者：
Crews, ST
中科院分区：
文献类型：
--
作者：
Kearney, JB;Wheeler, SR;Crews, ST
果蝇中枢神经系统中线细胞由中间神经元、运动神经元和神经胶质组成。CNS中线细胞作为研究CNS发育的神经基因组系统的效用来自于容易地鉴定CNS中线表达基因的能力。在这项研究中，我们使用了各种来源，以确定281个假定的中线表达基因，包括增强子陷阱线，微阵列数据，已发表的帐户，和伯克利果蝇基因组计划（BDGP）的基因表达数据。对于每个基因，我们分析了在胚胎中枢神经系统发育的所有阶段的表达，并对特定中线细胞类型的表达模式进行了分类。在281个候选基因中，我们鉴定了224个中线表达基因，其中包括转录因子、信号蛋白和转座元件。我们发现58个基因在中外胚层前体细胞中表达，138个在中线原基细胞中表达，143个在成熟中线细胞中表达，其中50个在中线胶质细胞中表达，106个在中线神经元中表达。此外，我们确定了27个基因表达的神经胶质细胞和中胚层细胞与中线细胞。这项工作为未来的研究提供了基础，这将产生一个完整的细胞和分子图谱的中枢神经系统中线发展，从而允许详细的遗传和分子研究神经元和神经胶质细胞的发展和功能。(C)2004爱思唯尔公司All rights reserved.
The Drosophila CNS midline cells constitute a specialized set of interneurons, motorneurons, and glia. The utility of the CNS midline cells as a neurogenomic system to study CNS development derives from the ability to easily identify CNS midline-expressed genes. For this study, we used a variety of sources to identify 281 putative midline-expressed genes, including enhancer trap lines, microarray data, published accounts, and the Berkeley Drosophila Genome Project (BDGP) gene expression data. For each gene, we analyzed expression at all stages of embryonic CNS development and categorized expression patterns with regard to specific midline cell types. Of the 281 candidates, we identified 224 midline-expressed genes, which include transcription factors, signaling proteins, and transposable elements. We find that 58 genes are expressed in mesectodermal precursor cells, 138 in midline primordium cells, and 143 in mature midline cells-50 in midline glia, 106 in midline neurons. Additionally, we identified 27 genes expressed in glial and mesodermal cells associated with the midline cells. This work provides the basis for future research that will generate a complete cellular and molecular map of CNS midline development, thus allowing for detailed genetic and molecular studies of neuronal and glial development and function. (C) 2004 Elsevier Inc. All rights reserved.