Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells.
Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells.
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牛乳头瘤病毒 E5 转化蛋白与转化小鼠细胞中活化的血小板衍生生长因子受体之间存在稳定关联。
DOI：
10.1073/pnas.89.15.6736
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发表时间：
1992
影响因子：
11.1
通讯作者：
DiMaio,D
中科院分区：
文献类型：
--
作者：
Petti,L;DiMaio,D
牛乳头瘤病毒E5转化蛋白由44个氨基酸组成，是目前已知的最短的诱导成纤维细胞致瘤转化的蛋白。我们先前已经证明，E5蛋白的表达激活了细胞中的血小板衍生生长因子(PDGF)的β受体，并提出激活的受体将转化信号传递给细胞。在此，我们使用免疫共沉淀分析表明，在转化的小鼠C127细胞中，E5蛋白和激活的PDGF受体以稳定的复合体存在。这些结果提示了一种不同的生长因子受体激活机制，并进一步证明PDGF受体是E5蛋白的重要靶点。
The 44-amino acid E5 transforming protein of bovine papillomavirus is the shortest protein known to induce tumorigenic transformation of fibroblasts. We showed previously that expression of the E5 protein activates the cellular beta receptor for platelet-derived growth factor (PDGF) and proposed that the activated receptor transmits the transforming signal to the cell. Here we use coimmunoprecipitation analysis to show that the E5 protein and the activated PDGF receptor exist in a stable complex in transformed mouse C127 cells. These results suggest a distinct mechanism of growth factor receptor activation and provide further evidence that the PDGF receptor is an important target of the E5 protein.