Structure of the human prealbumin gene.
Structure of the human prealbumin gene.
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人类前白蛋白基因的结构。
DOI：
10.1016/s0021-9258(17)39013-0
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
K. Shimada
中科院分区：
文献类型：
--
作者：
T. Tsuzuki;S. Mita;S. Maeda;S. Araki;K. Shimada
以克隆的人前白蛋白cDNA为探针，对人基因组DNA进行Southern印迹杂交，结果表明前白蛋白基因由一个独特的单拷贝DNA组成。测定了整个人前白蛋白基因的核苷酸序列，包括581个碱基对的5 ′-侧翼序列和95个碱基对的3 ′-侧翼序列。该基因跨越约7.0个内切酶对，由4个外显子和3个内含子组成。与大多数真核基因一样，共有TATA和CAAT序列分别位于推定帽位点上游的30和101个核苷酸处，并且在3 '非翻译区中发现多聚腺苷酸化信号序列AA-TAAA。出乎意料的是，在该基因内发现了两个独立的开放阅读框，其具有各自的调控序列：一个在第一内含子中，另一个在第三内含子中。
Using cloned human prealbumin cDNA as a probe, Southern blot hybridization of human genomic DNA revealed that the prealbumin gene consists of an unique, single-copy DNA. The nucleotide sequences of the entire human prealbumin gene, including both 581 base pairs of the 5'- and 95 base pairs of the 3'-flanking sequences, were determined. The gene spans about 7.0 kilobase pairs and consists of four exons and three introns. As in most eukaryotic genes, the consensus TATA and CAAT sequences are found 30 and 101 nucleotides, respectively, upstream from the putative cap site, and a polyadenylation signal sequence AA-TAAA is found in the 3'-untranslated region. Unexpectedly, two independent open reading frames provided with respective regulatory sequences were found within the gene: one in the first intron and the other in the third intron.