The adult central nervous cholinergic system of a neurogenetic model animal, the zebrafish Danio rerio
The adult central nervous cholinergic system of a neurogenetic model animal, the zebrafish Danio rerio
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DOI：
10.1016/j.brainres.2004.02.073
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发表时间：
2004-06-18
期刊：
影响因子：
2.9
通讯作者：
Wullimann, MF
中科院分区：
文献类型：
--
作者：
Mueller, T;Vernier, P;Wullimann, MF
本文报道了斑马鱼（Danio rerio）的中枢神经胆碱能系统，该系统是神经遗传学的一种模式动物，用免疫组织化学方法观察乙酰胆碱合成酶胆碱乙酰转移酶（ChAT）。含有ChAT的神经元胞体存在于端脑（腹侧端脑区的外侧核）、视前区（前/后小细胞和大细胞视前核）、间脑（缰核、背侧丘脑、后结节）、.中脑（Edinger-Westphal（EW）核、眼神经核、头侧被盖核、顶盖XIV型神经元）、峡部区域（瓣膜外侧核、次级味觉内脏感觉核、峡部核（NI）、周围神经系核、上级网状核（SRN））和菱形脑（三叉神经、外展神经、面部神经、舌咽-迷走神经运动神经核、八掌外侧肌传出神经元的吻侧和尾侧群体）。此外，在海马内也有一些ChAT阳性神经元。菱形脑网状结构、中央灰质和三叉神经降束旁细胞。在端脑腹侧区连合上核、端脑背侧区内侧带、顶盖前核浅小细胞、枕叶、八伏外侧内侧核、面叶、舌咽叶、迷走神经叶和下叶（侧隐窝室周核周围和弥漫核内）均有明显的ChAT阳性终末野。在此模型系统中提供的所有中枢神经胆碱能系统的鉴定对于它们的发育和维持的未来详细研究是关键的，例如，关于斑马鱼腹侧端脑和峡部上级网状神经元群体，可能分别代表哺乳动物的胆碱能基底前脑和脚桥/背侧被盖上行激活系统的至少一部分的同源物。(C)2004 Elsevier B. V.保留所有权利。
The central nervous cholinergic system of the zebrafish (Danio rerio), a model animal for neurogenetics, is documented here using inummohistochemical methods for visualizing choline acetyltransferase (ChAT), the acetylcholine synthesizing enzyme. Neuronal cell bodies containing ChAT are present in the telencephalon (lateral nucleus of ventral telencephalic area), preoptic region (anterior/posterior parvocellular and magnocellular preoptic nuclei), diencephalon (habenula, dorsal thalamus, posterior tuberculum),. mesencephalon (Edinger-Westphal (EW) nucleus, oculomotor nerve nucleus, rostral tegmental nucleus, tectal type XIV neurons), isthmic region (nucleus lateralis valvulae, secondary gustatory-viscerosensory nucleus, nucleus isthmi (NI), perilemniscal nucleus, superior reticular nucleus (SRN)) and rhombencephalon (trochlear, trigeminal, abducens, facial, glossopharyngeal-vagal motor nerve nuclei, rostral and caudal populations of octavolateralis efferent neurons). In addition, some ChAT positive neurons are present in. the rhombencephalic reticular formation, the central gray, and in cells accompanying the descending trigeminal tract. Obvious ChAT positive terminal fields are present in the supracommissural nucleus of area ventralis telencephali and the medial zone of area dorsalis telencephali, parvocellular superficial pretectal nucleus, torus semicircularis, medial octavolateralis nucleus, facial, glossopharyngeal, and vagal lobes, and in the inferior lobe (around the periventricular nucleus of the lateral recess and in the diffuse nucleus). The identification of all central nervous cholinergic systems provided here in this model system is pivotal for future detailed studies of their development and maintenance, e.g., with regard to the zebrafish ventral telencephalic and isthmic superior reticular neuronal populations, likely representing the homologues of at least part of the cholinergic basal forebrain and pedunculopontine/laterodorsal tegmental ascending activating systems of mammals, respectively. (C) 2004 Elsevier B.V. All rights reserved.