Identification and characterization of a low temperature regulated gene encoding an actin-binding protein from wheat
Identification and characterization of a low temperature regulated gene encoding an actin-binding protein from wheat
复制标题
DOI：
10.1016/0014-5793(96)00599-6
复制
发表时间：
1996-07-08
期刊：
影响因子：
3.5
通讯作者：
Sarhan, F
中科院分区：
文献类型：
--
作者：
Danyluk, J;Carpentier, E;Sarhan, F
从冷驯化的小麦cDNA文库中克隆了一个与肌动蛋白结合的蛋白的cDNA，命名为Wcor719，编码142个氨基酸，分子量为15.8kDa，等电点为4.27，该蛋白具有两个保守结构域，分别为肌动蛋白和磷脂酰肌醇4，北方分析表明，Wcor 719转录物的积累是快速的，并且受到低温的强烈上调，与不太耐受的物种相比，这种积累在耐受的冬小麦和黑麦物种中更大，该基因转录产物的快速诱导以及与不同生物体的肌动蛋白结合蛋白（ABP）的同源性表明，该基因的产物可能参与了低温驯化过程中细胞骨架的动态重组，也可能是低温驯化过程中信号转导途径中的一个关键因子。
A cDNA corresponding to a putative actin-binding protein was cloned from a cold-acclimated wheat cDNA library, The cDNA, designated Wcor719, encodes a polypeptide of 142 amino acids with a calculated molecular mass of 15.8 kDa and a pI of 4.27, The protein has the two conserved domains identified as actin and phosphatidylinositol 4,5-biphosphate (PIP2) binding sites found in members of the cofilin family, Northern analyses revealed that Wcor719 transcript accumulation is rapid and strongly up-regulated by low temperature, This accumulation was greater in the tolerant winter wheat and rye species compared to the less tolerant ones, The rapidity of transcript induction and the significant homology with actin-binding proteins (ABP) from different organisms suggest that the product of this gene might be involved in the dynamic reorganization of the cytoskeleton during low temperature acclimation, It may also serve as a key factor in the signal transduction pathway during cold acclimation.