In vivo cloning of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus genotypes.
In vivo cloning of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus genotypes.
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发表时间：
1998-03
影响因子：
5.5
通讯作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
中科院分区：
文献类型：
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作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
通过3龄棉铃虫低致死剂量感染，从野生型棉铃虫单核衣壳NPV （HaSNPV W）中分离出7个不同的基因型，并对从尸体中提取的原病毒dna进行了核酸内切酶分析。7个基因型各有不同的EcoRI谱。7个基因型的BamHI图谱可以通过一个特定的片段来区分：g1、g2、g3、g4dna的BamHI H片段大小为3.3kb， g5、g6的BamHI H片段大小为3.5kb， g7的BamHI H片段大小为2.7kb。这些结果表明，野生型HaSNPV是至少7个基因型变异的混合物。
Seven distinct genotypes were isolated from wild type Helicoverpa armigera single nucleocapsid NPV (HaSNPV W)by low mortality dose infection of 3rd instar H.armigera and progency viral DNAs extracted from individual cadaver were analysed by endonucleases.Each of the 7 genotypes had different EcoRI profiles.The BamHI profiles of the 7 genotypes could be distinguished by a particular fragment:the sizes of BamHI H fragment for G 1,G 2,G 3,G 4DNAs were 3.3kb,for G 5 and G 6,they were 3.5kb,and for G 7,it was 2.7kb.These results showed that the wild type HaSNPV was a mixture of at least 7 genotypic variants.