TGF-ALPHA AND TGF-BETA EXPRESSION IN MAMMARY-CARCINOMA
TGF-ALPHA AND TGF-BETA EXPRESSION IN MAMMARY-CARCINOMA
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DOI：
10.1002/path.1711700104
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发表时间：
1993-05-01
影响因子：
7.3
通讯作者：
LEVISON, DA
中科院分区：
文献类型：
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作者：
DUBLIN, EA;BARNES, DM;LEVISON, DA
采用兔多克隆抗体和免疫组化方法检测了195例乳腺癌组织中TGF α和TGF β的表达。在所有肿瘤中观察到TGF-α免疫反应性，但有9个，超过50%的染色强烈。多克隆TGF α抗体（CIM 1）与用于相同切片的市售小鼠单克隆TGF α抗体相比，具有良好的相关性（r = 0.52，P < 0.001）。两种TGF α抗体均产生颗粒状细胞质染色模式，其中CIM 1较粗，表明与聚集的蛋白质或细胞器结合。81%的肿瘤被TGF β抗体染色，35%被强烈染色。TGF-α和TGF-β的共表达有显著性差异（P < 0.001）。然而，他们没有被发现是有用的预后指标，缺乏任何显着的相关性与组织学分类，肿瘤大小，淋巴结状态，雌激素受体状态，S期分数，或总生存超过9-12年的时间。这些生长因子在大多数乳腺癌中的表达表明它们具有重要的生物学作用，但目前这些作用的确切性质仍不清楚。
TGF alpha and beta expression was examined using rabbit polyclonal antibodies and immunohistochemistry on a series of 195 breast carcinomas. TGF alpha immunoreactivity was observed in all but nine of the tumours, with over 50 per cent staining strongly. The polyclonal TGF alpha antibody (CIM 1), when compared with a commercially available mouse monoclonal TGF alpha antibody used on the same sections, gave a good correlation (r = 0.52, P < 0.001). Both TGF alpha antibodies produced a granular cytoplasmic staining pattern, that with CIM1 being coarser, suggestive of binding to an aggregated protein or organelle. Eighty-one per cent of tumours stained with the TGF beta antibody, 35 per cent strongly. There was significant co-expression of TGF alpha and TGF beta (P < 0.001). However, they were not found to be useful prognostic indicators, lacking any significant correlation with histological classification, tumour size, nodal status, oestrogen receptor status, S-phase fraction, or overall survival over a 9-12 year period. The expression of these growth factors in most breast carcinomas suggests that they have important biological roles, but the exact nature of these roles remains unclear at the moment.