BlastDigester - a web-based program for efficient CAPS marker design
BlastDigester - a web-based program for efficient CAPS marker design
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DOI：
10.1016/j.tig.2004.04.012
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发表时间：
2004-07-01
影响因子：
11.4
通讯作者：
Provart, NJ
中科院分区：
文献类型：
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作者：
Ilic, K;Berleth, T;Provart, NJ
单核苷酸多态性（SNP）和插入和缺失（InDels）是最常见的种内序列多态性类型，是创建用于基因分型的DNA标记的丰富资源。在这篇文章中，我们提出了一个基于网络的程序，用于生成切割扩增多态性序列（CAPS）标记，基于PCR的标记，使用多态性限制性内切酶消化模式（snip-SNP）。Blast-Digester通过计算“识别”Blast搜索中返回的序列比对，识别snip-SNP，并通过与引物设计程序Primer 3的动态链接简化PCR引物生成。
Single nucleotide polymorphisms (SNPs) and insertion and deletions (InDels), the most common type of intra-specific sequence polymorphism, are rich resources for creating DNA markers for genotyping. In this article, we present a web-based program for generating cleaved amplified polymorphic sequence (CAPS) markers, PCR-based markers that use polymorphism in restriction endonuclease digestion patterns (snip-SNPs). Blast-Digester computationally 'digests' the sequence alignments that are returned in a Blast search, identifies snip-SNPs and simplifies PCR-primer generation through dynamic links to Primer3, a primer design program.