DENDRITIC CELLS ARE CRITICAL ACCESSORY CELLS FOR THYMUS-DEPENDENT ANTIBODY-RESPONSES IN MOUSE AND IN MAN
DENDRITIC CELLS ARE CRITICAL ACCESSORY CELLS FOR THYMUS-DEPENDENT ANTIBODY-RESPONSES IN MOUSE AND IN MAN
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DOI：
10.1073/pnas.80.19.6041
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
MURAMATSU, S
中科院分区：
文献类型：
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作者：
INABA, K;STEINMAN, RM;MURAMATSU, S
树突状细胞（Dendritic cell，DC）是人和小鼠抗绵羊红细胞免疫反应中不可缺少的辅助细胞，在小鼠体内，少量DC（占培养物的0.3-1%）可使B/T淋巴细胞混合物的免疫反应恢复到正常脾的水平。如果T细胞已经针对抗原引发，则需要甚至更低的剂量（0.03- 0.1%DC）。应答是抗原和T细胞依赖性的。用单克隆抗体33 D1和补体从未分级脾选择性消耗DC消除抗体应答。与DC相反，纯化的脾巨噬细胞是弱的或无活性的刺激物。当与DC混合时，巨噬细胞可以使抗体分泌细胞的产量增加约2倍。在人类中，少量（0.3-1%）的血液DC在体外刺激抗体形成。纯化的人单核细胞不刺激，但在低剂量（培养物的1%）下抑制抗体应答。用抗体和补体选择性去除人单核细胞增强或加速抗体分泌细胞的发育。DC显然是体外小鼠和人淋巴细胞产生T依赖性抗体应答所必需的。
Dendritic cell (DC) are necessary and potent accessory cells for anti-sheep erythrocyte responses in both mouse and man. In mice, a small number of DC (0.3-1% of the culture) restores the response of B/T lymphocyte mixtures to that observed in unfractionated spleen. An even lower dose (0.03-0.1% DC) is needed if the T cells have been primed to antigen. Responses are both antigen and T cell dependent. Selective depletion of DC from unfractionated spleen with the monoclonal antibody 33D1 and complement ablates the antibody response. In contrast to DC, purified spleen macrophages are weak or inactive stimulators. When mixed with DC, macrophages can increase the yield of antibody-secreting cells about 2-fold. In man, small numbers (0.3-1%) of blood DC stimulate antibody formation in vitro. Purified human monocytes do not stimulate, but in low doses (1% of the culture) inhibit the antibody response. Selective removal of human monocytes with antibody and complement enhances or accelerates the development of antibody-secreting cells. DC are apparently required for the development of T-dependent antibody responses by mouse and human lymphocytes in vitro.