Messenger RNA competition in living Xenopus oocytes.
Messenger RNA competition in living Xenopus oocytes.
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活爪蟾卵母细胞中信使 RNA 的竞争。
DOI：
10.1111/j.1432-1033.1979.tb12892.x
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
H. Bloemendal
中科院分区：
文献类型：
--
作者：
F. Asselbergs;W. V. van Venrooij;H. Bloemendal
当小牛透镜晶状体蛋白mRNA和兔珠蛋白mRNA竞争活爪蟾卵母细胞中限制蛋白质合成的因素时，没有mRNA种类被优先选择用于翻译。mRNA种类的内在翻译效率存在差异，但相对效率在高和低mRNA浓度下是相同的。已经翻译的mRNA，特别是内源性卵母细胞mRNA，对注射mRNA的竞争性抑制不太敏感。随着注入的mRNA逐渐被整合到卵母细胞的蛋白质合成机制中，它们获得了与卵母细胞自身活性mRNA相同的状态。外源性mRNA变成内源性mRNA。这些结果，连同以前的估计的翻译效率的注射异源mRNA的物种，是兼容的假设，即大部分的内源性mRNA是不竞争的卵母细胞的翻译装置，因此，可能是存在于暂时失活的形式。
When calf lens crystallin mRNA and rabbit globin mRNA are competing for factors limiting protein synthesis in living Xenopus oocytes, no mRNA species is preferentially selected for translation. Differences in the intrinsic translational efficiency of the mRNA species exist, but the relative efficiencies are the same at high and low mRNA concentrations. mRNAs already being translated, in particular endogenous oocyte mRNAs, are less sensitive to competitive inhibition by injected mRNAs. As injected mRNAs gradually become incorporated into the protein-synthesizing machinery of the oocyte, they acquire the same status as the oocyte's own active mRNAs. Exogenous mRNAs this become endogenous mRNAs. These results, together with previous estmates of the translational efficiency of injected heterologous mRNA species, are compatible with the assumption that a large proportion of the endogenous mRNAs is not competing for the translational apparatus of the oocyte and, therefore, probably is present in the temporarily inactivated form.