Protective effects of recombinant staphylococcal enterotoxin type C mutant vaccine against experimental bovine infection by a strain of Staphylococcus aureus isolated from ubclinical mastitis in dairy cattle
Protective effects of recombinant staphylococcal enterotoxin type C mutant vaccine against experimental bovine infection by a strain of Staphylococcus aureus isolated from ubclinical mastitis in dairy cattle
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DOI：
10.1016/j.vaccine.2008.02.043
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发表时间：
2008-04-16
期刊：
影响因子：
5.5
通讯作者：
Park, Yong Ho
中科院分区：
文献类型：
--
作者：
Chang, Byoung Sun;Moon, Jin San;Park, Yong Ho
金黄色葡萄球菌是引起奶牛乳房炎的主要病原菌之一，然而，目前对牛金黄色葡萄球菌菌株有效的抗生素还不多见。aureus目前不可用。C型葡萄球菌肠毒素（SEC）是一种超抗原，是牛源葡萄球菌肠毒素中最常表达的一种。金黄色葡萄球菌和免疫原性决定因素之一。本研究的目的是评价重组SEC突变疫苗（MastaVaC（TM））对实验诱导的牛感染的保护效果。从对小鼠表现出不同致病强度的9个候选分离株中选择了3个代表性SEC分泌菌株，并以50-5.0 × 10（8）CFU/乳区的浓度接种到5头泌乳奶牛中。采用50 CFU的S.金黄色葡萄球菌409每季度，这是一个水平，这是不致命的小鼠。实验模型确定后，另外3头牛分别在第0天、2周和6周肌肉注射3剂疫苗，然后用S. 409. carter carter在免疫后4周进行的SEC特异性ELISA试验证实，所有接种牛中均存在高SEC抗体滴度。免疫组的体细胞数较低，而对照组的体细胞数在S.金黄色。攻毒后，S.在接种组中没有从任何牛中分离到金黄色葡萄球菌，而在对照组中从75%的牛中分离到金黄色葡萄球菌。上述结果表明，重组SEC突变体疫苗对沙门氏菌具有保护作用。奶牛乳房内感染金黄色葡萄球菌。(C)2008爱思唯尔有限公司保留所有权利。
Staphylococcus aureus is one of the main etiological agents of bovine mastitis; however, antibiotics that are effective against bovine strains of S. aureus are not currently available. Staphylococcal enterotoxin type C (SEC), a superantigen, is the enterotoxin most frequently expressed by bovine strains of S. aureus and one of immunogenic determinants. The purpose of this study was to evaluate the protective effectiveness of recombinant SEC mutant vaccine (MastaVaC (TM)) against experimentally induced bovine infection. Three representative SEC secreting strains were selected from 9 candidate isolates that showed various intensities of pathogenicity on mice and inoculated into 5 lactating dairy cattle at a concentration of 50-5.0 x 10(8) CFU per quarter. The optimal experimental bovine subclinical mastitis model was produced by inoculation with 50 CFU of S. aureus 409 per quarter, a level which was not lethal to mice. After the experimental model was determined, other 3 cattle were intramuscularly administered three doses of vaccine at day 0, at 2 wks and at 6 wks. Nine quarters of 3 vaccinated cattle and 8 quarters of 3 control cattle were then challenged with S. aureus 409. An SEC-specific ELISA test conducted at 4 wks post-immunization confirmed the presence of a high antibody titer against SEC in all vaccinated cattle. The somatic cell counts from the vaccinated group remained relatively low, whereas those of control group increased significantly after challenge with S. aureus. After challenge, S. aureus was not isolated from any cattle in the vaccinated group, whereas it was isolated from 75% of the cattle in the control group. These results indicate that recombinant SEC mutant vaccine had a protective effect against S. aureus intramammary infection in lactating cattle. (C) 2008 Elsevier Ltd. All rights reserved.