Hepatitis B virus DNA in Kaposi sarcoma.
Hepatitis B virus DNA in Kaposi sarcoma.
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卡波西肉瘤中的乙型肝炎病毒 DNA。
DOI：
10.1073/pnas.80.15.4861
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发表时间：
1983
影响因子：
11.1
通讯作者：
Siddiqui,A
中科院分区：
文献类型：
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作者：
Siddiqui,A
从Kaposi肉瘤活检组织中提取的DNA通过Southern杂交分析是否存在乙肝病毒(HBVDNA)序列。使用的探针是(I)分子克隆的HBVDNA亚基因组片段和(Ii)从Dane颗粒中提取的HBVDNA，其中包括编码乙肝表面抗原和核心抗原的DNA序列。本文报道的有限研究表明，在Kaposi肉瘤组织中，HBVDNA主要以异构体状态存在，并且这些DNA形式与在乙肝病毒粒子中发现的不同。多种DNA形式的存在表明，HBVDNA参与了DNA复制，这意味着该组织感染了HBV病毒。
DNA extracted from Kaposi sarcoma biopsies was analyzed for the presence of hepatitis B viral (HBV) DNA sequences by Southern blot hybridization. The probes used were (i) molecularly cloned subgenomic fragments of HBV DNA and (ii) HBV DNA extracted from Dane particles that included DNA sequences that encode hepatitis B surface antigen and core antigen. The limited study reported here demonstrates that HBV DNA exists mainly in an episomal state in the Kaposi sarcoma tissue and that these DNA forms are different from those found in hepatitis B virion. The presence of various DNA forms suggests that HBV DNA is engaged in DNA replication, which implies that the tissue is infected with HBV.