Induction of extremely low protein expression level by fusion of C-terminal region of Nef.
Induction of extremely low protein expression level by fusion of C-terminal region of Nef.
复制标题
通过 Nef C 末端区域的融合诱导极低的蛋白质表达水平。
DOI：
10.1002/bab.1021
复制
发表时间：
2012
影响因子：
2.8
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Takamune;N. *;Irisaka;Y.;Yamamoto;M.;Harada;K.;Shoji;S.;Misumi;S.
Nef是人类免疫缺陷病毒的辅助蛋白之一。在此，我们注意到NefNL 4 - 3在HEK 293细胞中的相对表达水平远低于NefJR-CSF。通过使用Nef突变体评估表达水平，表明Nef NL 4 - 3的氨基酸129 - 206，即命名为NLAA 129 - 206的C末端区域，可以包含负责诱导低蛋白表达水平的区域。此外，增强型绿色荧光蛋白和Renillaluciferase的表达水平随着NLAA 129 - 206的融合而变得极低。有趣的是，具有相对高表达水平的其他Nef变体的NLAA 129 - 206-对应序列也通过融合诱导极低的蛋白质表达水平。这些结果表明，Nef的C末端区域通常可以诱导极低的蛋白表达水平。在这里，我们提出Nef的C-末端区域可以成为一个很好的工具，用于通过连接作为融合蛋白诱导任意蛋白质的极低表达水平。
Nef is one of the accessory proteins of human immunodeficiency viruses. Here, we noted that the relative expression level of NefNL4‐3is much lower than that of NefJR‐CSFin HEK293 cells. By evaluating the expression level using a Nef mutant, it was indicated that amino acids 129‐206 of NefNL4‐3, that is, the C‐terminal region named NLAA129‐206, could contain the region responsible for the induction of the low protein expression level. In addition, the expression levels of the enhanced green fluorescent protein andRenillaluciferase became extremely low with the fusion of NLAA129‐206. Interestingly, the NLAA129‐206‐corresponding sequences of other Nef variants with relatively high expression levels also induced the extremely low protein expression level by fusion. These results suggest that the C‐terminal region of Nef can generally induce an extremely low protein expression level. Here, we propose that the C‐terminal region of Nef could become an excellent tool for the induction of an extremely low expression level of arbitrary proteins by attachment as fusion proteins.