Topoisomerase I regulates open chromatin and controls gene expression in vivo
Topoisomerase I regulates open chromatin and controls gene expression in vivo
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DOI：
10.1038/emboj.2010.109
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发表时间：
2010-07-07
期刊：
影响因子：
11.4
通讯作者：
Ekwall, Karl
中科院分区：
文献类型：
--
作者：
Durand-Dubief, Mickael;Persson, Jenna;Ekwall, Karl
DNA拓扑异构酶调节DNA双螺旋的拓扑状态，是DNA复制、转录和基因组稳定性过程中的关键酶。使用裂殖酵母模型粟酒裂殖酵母，我们调查全基因组DNA拓扑异构酶I和II如何影响体内染色质动力学和基因表达。我们表明，拓扑异构酶I活性是直接需要有效的核小体拆卸在基因启动子区域。拓扑异构酶活性的缺乏导致核小体占据增加，组蛋白修饰紊乱和这些启动子的转录减少。强相关的证据表明，拓扑异构酶I合作与ATP依赖的染色质重塑Hrp 1在核小体拆卸。我们的研究将拓扑异构酶活性与开放染色质的维持和体内转录调控联系起来。The EMBO Journal（2010）29，2126-2134. doi：10.1038/doj.2010.109; 2010年6月4日在线发布
DNA topoisomerases regulate the topological state of the DNA double helix and are key enzymes in the processes of DNA replication, transcription and genome stability. Using the fission yeast model Schizosaccharomyces pombe, we investigate genome wide how DNA topoisomerases I and II affect chromatin dynamics and gene expression in vivo. We show that topoisomerase I activity is directly required for efficient nucleosome disassembly at gene promoter regions. Lack of topoisomerase activity results in increased nucleosome occupancy, perturbed histone modifications and reduced transcription from these promoters. Strong correlative evidence suggests that topoisomerase I cooperates with the ATP-dependent chromatin remodeller Hrp1 in nucleosome disassembly. Our study links topoisomerase activity to the maintenance of open chromatin and regulating transcription in vivo. The EMBO Journal (2010) 29, 2126-2134. doi:10.1038/emboj.2010.109; Published online 4 June 2010