Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro
Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro
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DOI：
10.1002/term.359
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发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Bornhauser, Martin
中科院分区：
文献类型：
--
作者：
Goedecke, Anja;Wobus, Manja;Bornhauser, Martin
各种来源的间充质基质细胞（MSCs）在基于细胞的治疗中具有巨大的应用潜力。由于骨髓或脂肪组织中原代间充质干细胞的比例较低，因此在临床应用之前，需要进行塑料粘附和体外扩增来扩增间充质干细胞。人类富血小板血浆已被引入作为一种替代血清来源，但迄今为止尚未描述功能差异。在这里，我们在添加10%胎牛血清（FCS）或5%和10%富血小板血浆（PRP）的培养基中培养来自人骨髓的MSCs，直到第一次或第二次传代。研究的参数包括细胞产量、克隆原性、表型以及迁移和分化潜力。此外，我们还研究了不同血清来源对SDF-1 α分泌和CD34（+）造血干细胞（hsc）诱导迁移的影响。在第0代和第1代，PRP的使用显著提高了膨胀率和产量。此外，与人PRP相比，FCS培养的MSCs上清液中分泌的SDF-1 α水平显著升高。与此一致的是，在transwell实验中，用10% FCS培养的MSCs的迁移能力以及诱导CD34（+）造血祖细胞迁移的能力更高。我们的结果表明，人PRP可以被视为骨髓间充质干细胞培养中FCS的替代血清来源。然而，在选择各自的血清来源之前，必须仔细考虑具体临床应用的要求。版权所有John Wiley & Sons， Ltd。
Mesenchymal stromal cells (MSCs) derived from various sources have great potential for use in cell-based therapies. Since the proportion of primary MSCs contained in bone marrow or adipose tissue is low, plastic adherence and in vitro expansion are necessary to expand MSCs prior to clinical application. Human platelet-rich plasma has been introduced as an alternative serum source but functional differences have so far not been described. Here we cultured MSCs derived from human bone marrow in medium supplemented with either 10% fetal calf serum (FCS) or 5% and 10% platelet-rich plasma (PRP) until the first or second passage. Parameters under investigation were cell yield, clonogenicity, phenotype as well as migratory and differentiation potential. In addition, the secretion of SDF-1 alpha and the induced migration of CD34(+) haematopoietic stem cells (HSCs) were investigated with regard to the different serum source. The use of PRP resulted in a significantly higher expansion rate and yield at passages 0 and 1. In addition, the level of secreted SDF-1 alpha was significantly increased in the supernatant of MSCs cultured with FCS instead of human PRP. Consistent with this, the migration capacity of MSCs cultured with 10% FCS as well as their capability to induce the migration of CD34(+) haematopoietic progenitors in a transwell assay was higher. Our results demonstrate that human PRP can be seen as an alternative serum source to FCS for MSC cultivation. However, the requirements of the specific clinical application must be carefully considered before the respective serum source is selected. Copyright (C) 2010 John Wiley & Sons, Ltd.