Repeated stress increases catalytic TrkB mRNA in rat hippocampus
Repeated stress increases catalytic TrkB mRNA in rat hippocampus
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DOI：
10.1016/s0304-3940(99)00335-3
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发表时间：
1999-05-28
影响因子：
2.5
通讯作者：
Duman, RS
中科院分区：
文献类型：
--
作者：
Nibuya, M;Takahashi, M;Duman, RS
Northern blot分析利用转录物大小区分催化和截断的TrkB mRNA。重复（10天），但不是急性，固定应激显著增加催化TrkB mRNA水平，但不影响大鼠海马中截断TrkB转录本的表达。暴露于另一种模式，不同的，不可预测的压力源的组合，也增加了催化水平，但没有截断，TrkB mRNA。原位杂交分析表明，慢性应激可上调海马CA1和CA3锥体和齿状回颗粒细胞层的TrkB mRNA表达。正如之前报道的那样，急性和慢性固定应激都会降低BDNF mRNA的表达，这表明催化TrkB mRNA的上调可能是对重复应激的代偿性适应。1999爱思唯尔科学爱尔兰有限公司版权所有。
Northern blot analysis was utilized to distinguish between catalytic and truncated TrkB mRNA on the basis of transcript size. Repeated (10 days), but not acute, immobilization stress significantly increased levels of catalytic TrkB mRNA, but did not influence expression of truncated TrkB transcripts in rat hippocampus. Exposure to another paradigm, a combination of different, unpredictable stressors, also increased levels of catalytic, but not truncated, TrkB mRNA. In situ hybridization analysis demonstrated that chronic stress up-regulated TrkB mRNA in CA1 and CA3 pyramidal and dentate gyrus granule cells layers of hippocampus. As previously reported, both acute and chronic immobilization stress decreased expression of BDNF mRNA, suggesting that up-regulation of catalytic TrkB mRNA may be a compensatory adaptation to repeated stress. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.