Rapid and Ratiometric Fluorescent Detection of Cysteine with High Selectivity and Sensitivity by a Simple and Readily Available Probe
Rapid and Ratiometric Fluorescent Detection of Cysteine with High Selectivity and Sensitivity by a Simple and Readily Available Probe
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通过简单且易于使用的探针对半胱氨酸进行高选择性和高灵敏度的快速比例荧光检测
DOI：
10.1021/am505501d
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发表时间：
2014-10-22
影响因子：
9.5
通讯作者：
Feng, Guoqiang
中科院分区：
文献类型：
--
作者：
Liu, Yao;Yu, Dehuan;Feng, Guoqiang
我们报道了一种简单且易于获取的荧光探针，用于对具有生物学重要性的半胱氨酸（Cys）进行快速、特异和比率型荧光检测。该探针使用一种可见光激发的激发态分子内质子转移（ESIPT）染料（4'-二甲基氨基 - 3 - 羟基黄酮）作为荧光团，使用丙烯酸酯基团作为ESIPT阻断剂以及识别单元。在温和条件下，水溶液中的半胱氨酸能够特异且快速地裂解丙烯酸酯部分，这使得ESIPT过程得以恢复，并使该探针能够对半胱氨酸呈现出快速、比率型的荧光检测过程，且对包括同型半胱氨酸（Hcy）和谷胱甘肽（GSH）在内的各种分析物具有高选择性。该探针对半胱氨酸的检测限约为0.2 μM，并且该探针对细胞内半胱氨酸的生物成像在活细胞中成功应用，这表明该探针在生物应用方面具有巨大潜力。
We report a simple and readily available fluorescent probe for rapid, specific, and ratiometric fluorescent detection of the biologically important cysteine (Cys). This probe uses a visible-light excitable excited-state intramolecular proton transfer (ESIPT) dye (4'-dimethylamino-3-hydroxyflavone) as the fluorophore and an acrylate group as the ESIPT blocking agent as well as the recognition unit. Cleavage of the acrylate moiety can be achieved specifically and rapidly by Cys in aqueous solution under mild conditions, which leads to restore the ESIPT process and enables the probe to show a rapid, ratiometric fluorescent detection process for Cys with high selectivity over various analytes, including homocysteine (Hcy) and glutathione (GSH). The detection limit of this probe for Cys was found to be similar to 0.2 mu M and bioimaging of intracellular Cys by this probe was successfully applied in living cells, indicating that this probe holds great potential for biological applications.