Improvement of quality control for steroid receptor measurements: Analysis of distributions in more than 40000 primary breast cancers
Improvement of quality control for steroid receptor measurements: Analysis of distributions in more than 40000 primary breast cancers
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DOI：
10.1007/bf01807158
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发表时间：
1996-01-01
影响因子：
3.8
通讯作者：
Magdelenat, H
中科院分区：
文献类型：
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作者：
Romain, S;Spyratos, F;Magdelenat, H
所有常规测定雌二醇（ER）和孕酮（PR）受体的法国实验室都参与了欧洲EORTC质量控制计划，该计划基于每年两次对5种细胞溶质制剂的分析。该系统大大减少了实验室间的差异，但并不涵盖这些测定的所有方面。受体值分布的分析对于确保受体测量随时间保持稳定也至关重要，与实验室和测定方法无关。这项研究涉及83907受体测定在过去的17年中进行的17个实验室属于法国组织和分子生物病理学研究组。这些测定是基于放射性配体结合（RLA）或免疫酶学（EIA）。对于每个实验室，中位数和阳性率进行了分析，根据两个完全客观的标准，患者的年龄和检测年份，并根据组织学分级和组织学类型的肿瘤，以验证经典描述的相关性。与7个欧洲EORTC实验室发表的数据相比，实验室之间的ESTA相关分布变化不大[1]。ER和PR的RLA方法和PR的EIA方法的结果随着时间的推移保持相对稳定。ER-EIA数据的中位数在1987年至1989年期间显著增加，主要是由于Abbott试剂在此期间的质量变化。否则，该分析证实了先前的病理生理学观察结果。
All French laboratories that routinely assay estradiol (ER) and progesterone (PR) receptors participate in the European EORTC quality control program based on twice-yearly analysis of 5 cytosolic preparations. This system has considerably reduced inter-laboratory variations, but does not cover all aspects of these assays. Analysis of receptor value distributions is also crucial to ensure that receptor measurements remain stable with time, independently of the laboratory and assay method. This study involved 83907 receptor assays carried out in the last 17 years by 17 laboratories belonging to the French Study Group on Tissue and Molecular Biopathology. The assays were based on radioligand binding (RLA) or immunoenzymology (EIA). For each laboratory, the medians and positivity rates were analysed according to two totally objective criteria, the patient's age and the year of assay, and according to histological grade and histological type of the tumor in order to verify the correlations classically described. Age-related distributions varied little between laboratories, compared with data published by 7 European EORTC laboratories [1]. The results remained relatively stable with time in the RLA method for ER and PR, and in the EIA method for PR. Median ER-EIA data showed a marked increase between 1987 and 1989, mainly due to changes in the quality of Abbott reagents during this period. Otherwise, this analysis confirms previous pathophysiological observations.