Mechanism on insulin-like action of vanadyl sulfate: studies on interaction between rat adipocytes and vanadium compounds.
Mechanism on insulin-like action of vanadyl sulfate: studies on interaction between rat adipocytes and vanadium compounds.
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硫酸氧钒的胰岛素样作用机制：大鼠脂肪细胞与钒化合物相互作用的研究。
DOI：
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发表时间：
1995
影响因子：
2
通讯作者：
H. Sakurai
中科院分区：
文献类型：
--
作者：
M. Nakai;H. Watanabe;Chikako Fujiwara;H. Kakegawa;T. Satoh;J. Takada;R. Matsushita;H. Sakurai
给链脲佐菌素(STZ)诱导的糖尿病大鼠每日一次腹腔注射(Ip)。注射VOSO4(钒的+4氧化态)后，血糖在2d内从高血糖降至正常水平，血清游离脂肪酸(FFA)水平也降至正常水平。中子活化分析(NAA)检测到，钒在大多数器官和脂肪组织中都有掺入。本文探讨了钒对大鼠脂肪细胞释放游离脂肪酸的胰岛素样作用机制：(1)钒(IV)和钒(III)离子使肾上腺素处理的脂肪细胞FFA释放正常化：(2)抗坏血酸、半胱氨酸或葡萄糖处理的钒(V)离子能有效地使FFA释放正常化，但单独钒离子对FFA释放无影响；(3)如ESR波谱所示，钒离子进入脂肪细胞，而钒离子不影响FFA释放；以及(4)钒离子可以作用于葡萄糖转运蛋白，通过使用细胞松弛素B的实验表明，细胞松弛素B是葡萄糖转运蛋白的抑制剂。这些结果表明，钒离子对血清葡萄糖和游离脂肪酸水平的正常化是由于钒离子掺入脂肪细胞内，金属离子作用于葡萄糖转运体，导致外周脂肪细胞对葡萄糖的摄取促进和游离脂肪酸释放减少。钒的状态被认为是钒的一种可能的药理活性形式，允许胰岛素样的作用。
When rats with streptozotocin (STZ)-induced diabetes were given a daily intraperitoneal (i.p.) injection of VOSO4 (+4 oxidation state of vanadium), their serum glucose dropped from hyperglycemic level to normal level within 2d and serum free fatty acid (FFA) level also dropped to normal level. Vanadium was incorporated in most organs as well as in the adipose tissues, as detected by neutron activation analysis (NAA). The mechanism for the insulin-like action vanadium in terms of FFA release from isolated rat adipocytes was investigated: (1) Vanadyl (IV) and vanadic (III) ions normalize the FFA release in the adipocytes treated with epinephrine; (2) vanadate (V) ion treated with ascorbic acid, cysteine or glucose is effective in normalizing the FFA release but vanadate ion alone has no effect on FFA release; (3) vanadyl ion is incorporated into the adipocytes, while vanadate ion is not, as indicated by ESR spectroscopy; and (4) vanadyl ion can act on the glucose transporter, as indicated by experiments using cytochalasin B which is an inhibitor of this transporter. From these results, the normalization of both serum glucose and FFA levels by vanadyl ion was concluded to be due to the incorporation of vanadyl ion into the adipocytes, in which the metal ion acts on the glucose transporter and induces both the promotion of glucose uptake and the decrease of FFA release form peripheral adipocytes. The vanadyl state was suggested to be a possible pharmacologically active form of vanadium allowing the insulin-like action.(ABSTRACT TRUNCATED AT 250 WORDS)