Prophylactic fenbendazole therapy does not affect the incidence and onset of type 1 diabetes in non-obese diabetic mice.
Prophylactic fenbendazole therapy does not affect the incidence and onset of type 1 diabetes in non-obese diabetic mice.
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预防性芬苯达唑治疗不会影响非肥胖糖尿病小鼠 1 型糖尿病的发病率和发作。
DOI：
10.1093/intimm/dxh385
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Shirwan,Haval
中科院分区：
文献类型：
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作者：
Franke,DeannaDH;Shirwan,Haval
芬苯达唑(FBZ)是一种常见、高效的广谱驱虫药，用于治疗和限制啮齿动物蛔虫感染。然而，预防性使用它对啮齿动物免疫反应的影响在很大程度上是不确定的。非肥胖糖尿病(NOD)小鼠是研究1型糖尿病(T1D)的常用模型。寄生虫感染将抑制NOD小鼠糖尿病的发展；因此，在存在污染的情况下，必须考虑使用驱虫药进行预防性治疗，以保持实验研究。在此，我们研究了FBZ在NOD小鼠中的预防性使用，以确定其对糖尿病的发生和发病、淋巴细胞亚群和T细胞增殖反应的影响。NOD小鼠随机分为对照组和治疗组。治疗组给予含FBZ的饮食。监测动物T1D的发病率和发病情况。在匹配的时间点，糖尿病和非糖尿病小鼠被处死，并用流式细胞术分析不同细胞亚群和丝裂原诱导的增殖反应的脾淋巴细胞。治疗组和对照组的小鼠被监测了23周，对糖尿病的发病率或发病没有可检测到的影响。此外，在对照组和治疗组之间，淋巴细胞亚群和丝裂原诱导的CD4+和CD8+增殖反应没有显著差异。这些结果表明，预防性的FBZ治疗不会显著改变NOD小鼠的糖尿病发病率或发病。因此，预防性使用FBZ为预防珍贵实验动物的蛔虫感染提供了一种可行的方法，具有可观的科学和经济效益。
Fenbendazole (FBZ) is a common, highly efficacious broad-spectrum anthelmintic drug used to treat and limit rodent pinworm infections. However, the effect of its prophylactic use on the immune response of rodents is largely undefined. The non-obese diabetic (NOD) mouse is a model commonly used to study type 1 diabetes (T1D). Parasitic infections will inhibit diabetes development in NOD mice; thus, in the presence of contamination, prophylactic treatment with anthelmintics must be considered to maintain experimental research. Herein, we investigated the prophylactic use of FBZ in NOD mice to determine its effect on the incidence and onset of diabetes, lymphocyte sub-populations and T cell proliferative responses. NOD mice were separated into control and treatment groups. The treatment group received a diet containing FBZ. Animals were monitored for the incidence and onset of T1D. At matched time points, diabetic and non-diabetic mice were killed and splenic lymphocytes analyzed for various cell sub-populations and mitogen-induced proliferative responses using flow cytometry. Treated and control mice were monitored >23 weeks with no detectable effects on the incidence or onset of diabetes. Moreover, no significant differences were detected in lymphocyte sub-populations and mitogen-induced CD4+and CD8+proliferative responses between control and treatment groups. These results suggest that prophylactic FBZ treatment does not significantly alter the incidence or onset of diabetes in NOD mice. The prophylactic use of FBZ, therefore, presents a viable approach for the prevention of pinworm infection in precious experimental animals with substantial scientific and economic benefits.