The Soybean β-Conglycinin β 51–63 Fragment Suppresses Appetite by Stimulating Cholecystokinin Release in Rats
The Soybean β-Conglycinin β 51–63 Fragment Suppresses Appetite by Stimulating Cholecystokinin Release in Rats
复制标题
大豆 β-伴大豆球蛋白 β 51–63 片段通过刺激大鼠胆囊收缩素释放来抑制食欲
DOI：
10.1093/jn/133.8.2537
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发表时间：
2003
影响因子：
4.2
通讯作者：
F. Tomita
中科院分区：
文献类型：
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作者：
T. Nishi;H. Hara;Kozo Asano;F. Tomita
我们之前证明，大豆β-伴大豆球蛋白蛋白胨通过直接作用于大鼠小肠粘膜细胞刺激胆囊收缩素（CCK）释放来抑制食物摄入和胃排空。本研究的目的是通过使用从三个 β-伴大豆球蛋白亚基序列中选择的合成肽来定义 β-伴大豆球蛋白中的活性片段。我们选择了具有多个不相邻精氨酸残基的片段，并研究了它们与大鼠肠刷状缘膜成分结合以及刺激 CCK 释放和食欲抑制的能力。 β亚基的51至63片段（β 51-63）具有最强的结合活性。十二指肠内输注 β 51-63 可抑制食物摄入并显着增加门脉 CCK 浓度。 β 51-63 影响食物摄入的阈值浓度为 3 micromol/L。 CCK-A 受体拮抗剂消除了 β 51-63 诱导的食物摄入抑制。 β 51-63 的三种较小片段（β 51-59、β 53-63 和 β 53-59）以及 β-伴大豆球蛋白 α 和 α' 亚基中类似于 β 51-63 的两种类型的片段（α 212-224 和 α' 230-240）具有比 β 51-63 更低的结合能力。由精氨酸（R）和甘氨酸（G）构建的模型肽，例如GRGRGRG，具有很强的结合亲和力，但含有单个R或RR的肽则没有。这些结果表明，β-伴大豆球蛋白β 51-63 片段是β-伴大豆球蛋白中的生物活性食欲抑制剂，并且该片段中的多个精氨酸残基可能参与了这种作用。
We previously demonstrated that soybean beta-conglycinin peptone suppresses food intake and gastric emptying by direct action on rat small intestinal mucosal cells to stimulate cholecystokinin (CCK) release. The aim of the present study was to define the active fragment in beta-conglycinin by using synthetic peptides chosen from the sequence of three beta-conglycinin subunits. We selected the fragments that had multiple nonadjacent arginine residues, and investigated their ability to bind to components of the rat intestinal brush border membrane as well as to stimulate CCK release and appetite suppression. The fragment from 51 to 63 of the beta subunit (beta 51-63) had the strongest binding activity. Intraduodenal infusion of beta 51-63 inhibited food intake and markedly increased portal CCK concentration. The threshold concentration of beta 51-63 to affect food intake was 3 micro mol/L. The CCK-A receptor antagonist abolished the beta 51-63-induced suppression of food intake. Three types of smaller fragments of beta 51-63 (beta 51-59, beta 53-63 and beta 53-59) and two types of fragments similar to beta 51-63 in the beta-conglycinin alpha and alpha' subunits (alpha 212-224 and alpha' 230-240) had less binding ability than did beta 51-63. Model peptides constructed with arginine (R) and glycine (G), such as GRGRGRG, had strong binding affinity, but peptides containing a single R or RR did not. These results indicate that the beta-conglycinin beta 51-63 fragment is the bioactive appetite suppressant in beta-conglycinin, and multiple arginine residues in the fragment may be involved in this effect.
影响因子：
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作者：
LIDDLE, RA;GREEN, GM;WILLIAMS, JA
通讯作者：
WILLIAMS, JA
胆囊收缩素在大鼠十二指肠输送蛋白胨引起的厌食中的作用。
DOI：
10.1152/ajpregu.1999.276.6.r1701
发表时间：
1999
期刊：
The American journal of physiology.
影响因子：
--
作者：
Woltman,T;Reidelberger,R
通讯作者：
Reidelberger,R