AUTONOMOUSLY REPLICATING SEQUENCES IN SACCHAROMYCES-CEREVISIAE
AUTONOMOUSLY REPLICATING SEQUENCES IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.77.11.6329
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
TYE, BK
中科院分区：
文献类型：
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作者：
CHAN, CSM;TYE, BK
提出了一种从S.基于自主复制质粒和非复制质粒的不同转化能力，能够在酵母中自我复制的DNA质粒以比非复制质粒高约1000倍的频率转化酵母原生质球。从总酵母DNA中克隆了许多DNA片段，这些片段允许携带酵母LEU 2基因的pBR 322质粒自主复制。这些质粒克隆的特征在于它们以高频率将Leu-原生质球转化为Leu+的能力以及它们自主复制的能力。对这些自我复制质粒中携带的插入DNA的分析将它们分为两类：那些在酵母基因组中是独特的和那些是重复的。
A method is presented for isolating DNA segments capable of autonomous replication from S. cerevisiae chromosomal DNA based on the differential transforming ability of autonomously replicating plasmids and nonreplicating plasmids. DNA plasmids that are capable of self-replication in yeast transform yeast spheroplasts at about 1000-fold higher frequency than nonreplicating plasmids. From total yeast DNA a number of DNA segments were cloned that permit the pBR322 plasmid carrying the yeast LEU2 gene to replicate autonomously. These plasmid clones are characterized by their ability to transform Leu- spheroplasts to Leu+ at a high frequency and their ability to replicate autonomously. Analysis of the insert DNA carried in some of these self-replicating plasmids divides them into 2 categories: those that are unique in the yeast genome and those that are repetitive.