Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells.
Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells.
复制标题
应用光漂白测量酵母细胞胞浆中朊病毒蛋白的扩散。
DOI：
10.1016/j.ymeth.2006.04.004
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Greene,LoisE
中科院分区：
文献类型：
--
作者：
Wu,Yue-Xuan;Masison,DanielC;Eisenberg,Evan;Greene,LoisE
Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intensity laser light irreversibly bleaches the GFP fluorescence but has no effect on protein function. By photobleaching a limited region of the cytoplasm, the rate of fluorescence recovery provides a measure of the rate of protein diffusion. A detailed description of the FRAP technique is given, including its application to measuring the mobility of GFP-tagged Sup35p in [psi−] and [PSI+] cells.
