High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.
High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.
复制标题
DOI：
10.1007/978-1-0716-0644-5_34
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jinks-Robertson S
中科院分区：
文献类型：
--
作者：
Gamble D;Hum YF;Jinks-Robertson S
有丝分裂双链断裂 (DSB) 通过与同源供体双链体重组来修复。该过程涉及断裂分子和修复模板之间单链 DNA 的交换，从而产生异源双链 DNA (hetDNA) 区域。定义的 DSB 的创建与序列分歧修复模板的使用相结合，可以通过重组产物的测序来进行 hetDNA 的精细结构映射。描述了一种利用 PacBio 开发的单分子实时 (SMRT) 测序技术的高通量方法。该方法可以同时分析数百种重组产物中包含的 hetDNA。
Mitotic double-strand breaks (DSBs) are repaired by recombination with a homologous donor duplex. This process involves the exchange of single DNA strands between the broken molecule and the repair template, giving rise to regions of heteroduplex DNA (hetDNA). The creation of a defined DSB coupled with the use of a sequence-diverged repair template allows the fine-structure mapping of hetDNA through the sequencing of recombination products. A high-throughput method is described that capitalizes on the single-molecule real-time (SMRT) sequencing technology developed by PacBio. This method allows simultaneous analysis of the hetDNA contained within hundreds of recombination products.