Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions
Valence-driven colorimetric detection of norovirus protease via peptide-AuNP interactions
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DOI：
10.1039/d3cc04142e
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发表时间：
2023-09-25
影响因子：
4.9
通讯作者：
Jokerst，Jesse V.
中科院分区：
文献类型：
--
作者：
Ling，Chuxuan;Jin，Zhicheng;Jokerst，Jesse V.
我们在这里报告了一种快速检测诺如病毒的比色方法的基础上的价驱动的肽-金纳米粒子的相互作用。我们设计了一个名为K1的肽序列，在两个赖氨酸残基之间具有切割序列。带正电荷的赖氨酸基团聚集带负电荷的纳米颗粒，导致紫色变化。当切割序列被南安普顿诺如病毒3C样蛋白酶（SV 3CP）--一种参与人诺如病毒（HNV）生命周期的蛋白酶消化时，存在红色。在Tris缓冲液中测定的检测限为320 nM。我们进一步表明，该传感器在呼出气冷凝物，尿液和粪便物质中具有良好的性能。本研究为选择性检测HNV提供了一种潜在的简便快速的方法。
We report here a colorimetric method for rapid detection of norovirus based on the valence-driven peptide-AuNP interactions. We engineered a peptide sequence named K1 with a cleavage sequence in between two lysine residues. The positively charged lysine groups aggregated the negatively charged nanoparticles leading to a purple color change. There was a red color when the cleavage sequence was digested by the Southampton norovirus 3C-like protease (SV3CP)—a protease involved in the life cycle of Human norovirus (HNV). The limit of detection was determined to be 320 nM in Tris buffer. We further show that the sensor has good performance in exhaled breath condensate, urine, and faecal matter. This research provides a potential easy and quick way to selectively detect HNV.