The Acyltransferase Gene bus-1 Exhibits Conserved and Specific Expression in Nematode Rectal Cells and Reveals Pathogen-Induced Cell Swelling
The Acyltransferase Gene bus-1 Exhibits Conserved and Specific Expression in Nematode Rectal Cells and Reveals Pathogen-Induced Cell Swelling
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DOI：
10.1002/dvdy.21792
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发表时间：
2008-12-01
影响因子：
2.5
通讯作者：
Hodgkin, Jonathan
中科院分区：
文献类型：
--
作者：
Gravato-Nobre, Maria J.;Hodgkin, Jonathan
对直肠病原体嗜热微杆菌的敏感性提供了一种检查C.优雅bus-1的突变体对这种病原体的感染具有抗性。我们在这里显示，总线-1编码预测的酰基转移酶表达在直肠上皮细胞（K，F和U），这表明其参与区域表面修饰。Bus-1报告基因用于显示后肠发育控制基因EGL-38、mAb-9和mAb-23的空间调节。Bus-1：：GFP报告基因揭示了M.喜食草梭Bus-1的Briggsae直系同源物显示保守的功能和直肠表达，但其在外阴和直肠细胞中表达，这与该物种中病原体对外阴和直肠细胞的粘附相关。另一种影响细菌粘附的酰基转移酶bus-18/acl-10也被鉴定，其也显示出强直肠表达，但其在另外的上皮组织中表达，并且是一般表面完整性所需的。发展动力学237：3762-3776，2008年。(c)2008 Wiley-Liss，Inc.
Susceptibility to the rectal pathogen Microbacterium nematophilum provides a means of examining hindgut differentiation in C. elegans. Mutants of bus-1 are resistant to infection with this pathogen. We show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (K, F, and U), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. A bus-1::GFP reporter reveals the conspicuous rectal epithelial swelling induced by M. nematophilum. The C. briggsae ortholog of bus-1 exhibits conserved function and rectal expression, but it is expressed in vulval as well as rectal cells, correlated with pathogen adhesion to both vulval and rectal cells in this species. Another acyltransferase affecting bacterial adhesion, bus-18/acl-10, was also identified, which also shows strong rectal expression, but it is expressed in additional epithelial tissues and is required for general surface integrity. Developmental Dynamics 237:3762-3776, 2008. (c) 2008 Wiley-Liss, Inc.