Development of a dual-wavelength fluorescent nanoprobe for in vivo and in vitro cell tracking consecutively.
Development of a dual-wavelength fluorescent nanoprobe for in vivo and in vitro cell tracking consecutively.
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DOI：
10.1016/j.bmc.2019.03.036
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发表时间：
2019-05
影响因子：
3.5
通讯作者：
Hong Vu;Jun Zhou;YiHui Huang;Amirhossein Hakamivala;M. Khang;Liping Tang
中科院分区：
文献类型：
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作者：
Hong Vu;Jun Zhou;YiHui Huang;Amirhossein Hakamivala;M. Khang;Liping Tang
许多成像探头已经开发出各种各样的成像模式。然而，没有一种光学成像探针可以同时用于动物的显微和全身成像。为了填补这一空白，研制了双波长荧光成像纳米探针，可以同时携带可见光范围的荧光染料和近红外（NIR）染料。发射扫描证实，纳米探针在可见光和近红外范围内具有两个独立的峰，具有很强的荧光强度。此外，该双波长荧光纳米探针具有较高的光稳定性和胶体稳定性，并且具有较长的保质期。体外细胞培养实验表明，纳米探针能够标记不同类型的细胞（即食管细胞、前列腺细胞、成纤维细胞和巨噬细胞）进行荧光显微镜成像。更重要的是，细胞跟踪实验证实，分别使用体内全身近红外成像和体外显微成像可以实时跟踪细胞在各器官中的迁移和分布。
Many imaging probes have been developed for a wide variety of imaging modalities. However, no optical imaging probe could be utilized for both microscopic and whole animal imaging. To fill the gap, the dual-wavelength fluorescent imaging nanoprobe was developed to simultaneously carry both visible-range fluorescent dye and near-infrared (NIR) dye. Emission scan confirms that the nanoprobe exhibits two separate peaks with strong fluorescent intensity in both visible and NIR ranges. Furthermore, the dual-wavelength fluorescent nanoprobe has high photostability and colloidal stability, as well as long shelf-life. In vitro cell culture experiments show that the nanoprobe has the ability to label different types of cells (namely, esophageal, prostate, fibroblast and macrophage cell) for fluorescent microscope imaging. More importantly, cell tracking experiments confirm that cell migration and distribution in various organs can be tracked in real time using in vivo whole-body NIR imaging and in vitro microscopic imaging, respectively.