Collateralization of climbing and mossy fibers projecting to the nodulus and flocculus of the rat cerebellum
Collateralization of climbing and mossy fibers projecting to the nodulus and flocculus of the rat cerebellum
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DOI：
10.1002/cne.10889
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发表时间：
2003-11-10
影响因子：
2.5
通讯作者：
Ruigrok, TJH
中科院分区：
文献类型：
--
作者：
Ruigrok, TJH
用霍乱毒素b亚单位在大鼠前庭小脑皮质注射的方法，观察苔藓纤维和爬行纤维的侧支作用。注射的特点是在下橄榄内逆行标记。侧支标记是使用颜色编码的整个小脑皮质的密度分布绘制的。在结节的内侧部分注射导致橄榄色标记，仅限于背帽的吻部。内侧、外侧结节带、腹侧小叶旁及邻近小叶均可见攀缘纤维侧支。在结节的中间部分注射可导致P亚核的橄榄状标记，但也可累及腹外侧突。在后一种情况下，在两个绒毛区和在I小腿最外侧的一小部分可见攀缘纤维侧枝。所有结节注射均显示两侧对称分布的苔藓纤维花环，主要局限于前庭小脑，主要起源于前庭核团。小叶内的注射标记了背帽的尾部和/或腹外侧突。苔藓纤维花环遍及前庭小脑，但也包括小脑皮质的其他区域，呈两侧对称的模式，与更广泛的小脑前起源相对应。起源于吻侧背帽的攀升纤维，从腹侧旁丘脑注射而标记，侧支至结节的内侧和外侧区域。攀缘纤维侧支通常伴有苔藓纤维花环的标记。这些结果表明，一些结节带和絮状带是相关的，至少部分地共享共同的输入。(C)2003年Wiley-Liss，Inc.
Collateralization of mossy and climbing fibers was investigated using cortical injections of cholera toxin b-subunit in the rat vestibulocerebellum. Injections were characterized by their retrograde labeling within the inferior olive. Collateral labeling was plotted using color-coded density profiles of the whole cerebellar cortex. Injections in the medial part of the nodulus resulted in olivary labeling that was restricted to the rostral part of the dorsal cap. Climbing fiber collaterals were found in medial and lateral nodular zones as well as in the ventral paraflocculus and adjacent flocculus. Injections in the intermediate part of the nodulus resulted in olivary labeling of the P-subnucleus but could also involve the ventrolateral outgrowth. In the latter case, climbing fiber collaterals were found in the two floccular zones and in a small region in the lateral-most part of crus I. All nodular injections showed a bilaterally symmetric distribution of collateral mossy fiber rosettes that was mostly confined to the vestibulocerebellum and originated predominantly from the vestibular nuclei. Injections in the flocculus labeled the caudal part of the dorsal cap and/or the ventrolateral outgrowth. Mossy fiber rosettes were observed throughout the vestibulocerebellum but also included other regions of the cerebellar cortex in a bilaterally symmetric pattern corresponding with a more widespread precerebellar origin. Climbing fibers originating in the rostral dorsal cap, labeled from an injection in the ventral paraflocculus, collateralize to a medial and lateral zone in the nodulus. Climbing fiber collaterals were usually accompanied by subjacent labeling of mossy fiber rosettes. These results demonstrate that some nodular and floccular zones are related and, at least partially, share a common input. (C) 2003 Wiley-Liss, Inc.